- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 10 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Mouse IL-12/IL-23(p40) ELISA Test Kit is designed for quantitative determination of the concentration of mouse IL-12/IL-23(p40) in serum, plasma, and cell culture supernatant.
Biologically active mouse IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein that is composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine which belongs to the IL-12 cytokine family. IL-12 and IL-23 are important immunoregulatory molecules. They share overlapping but distinct biological activities, which promote cell-mediated immunity. Interleukin-12 (IL-12) is a heterodimeric pleiotropic cytokine that is primarily produced by monocytes, macrophages, and dendritic cells in response to bacterial lipopolysaccharides (LPS), to intracellular pathogens, and to activated T cells. IL-23 is an important part of the inflammatory response against infection. IL-23 promotes up-regulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T cell infiltration. In addition to being secreted as a component of IL-12 or IL-23, free p40 monomers and homodimers are also secreted.This ELISA kit specifically measures the mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer.
Format: 96-well strip plate
Assay range: 10-640 pg/mL
Sensitivity: 10 pg/mL
Total Assay Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm