NEXTflex® Rapid DNA-Seq Kit 2.0

Library prep for sequencing on Illumina® platforms

Catalog# Product Name Quantity US List Price
NOVA-5188-01 NEXTflex® Rapid DNA-Seq Kit 2.0 8 rxns $194 Buy Now
NOVA-5188-02 NEXTflex® Rapid DNA-Seq Kit 2.0
48 rxns $1132 Buy Now
NOVA-5188-03 NEXTflex® Rapid DNA-Seq Kit 2.0
96 rxns $2208 Buy Now
NOVA-5188-11 NEXTflex® Rapid DNA-Seq Kit 2.0 Bundle with NEXTflex-HT™ Barcodes 1 - 8 8 rxns $232 Buy Now
NOVA-5188-12 NEXTflex® Rapid DNA-Seq Kit 2.0 Bundle with NEXTflex-HT™ Barcodes 1 - 48
48 rxns $1353 Buy Now
NOVA-5188-13 NEXTflex® Rapid DNA-Seq Kit 2.0 Bundle with NEXTflex-HT™ Barcodes 1 - 96 96 rxns $2640 Buy Now

NEXTFLEX Rapid DNA-Seq Kit 2.0

  • Complete library prep solution, including size selection beads
  • Bundle kits available with library prep reagents and barcodes
  • Up to 384 barcodes available upon request
  • Convenient and cost-effective package in a 8, 48, or 96 reaction format
  • Simplified workflow
  • Automation compatible with liquid handlers, including the PerkinElmer® Sciclone® G3 NGS workstation
  • Robust genome coverage
  • Reliable performance with GC-bias and sequencing bias mitigation 
  • Low input requirement for genomic DNA down to 1 ng
  • Functionally validated with Illumina® sequencing platforms

 

The NEXTflex® rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms. The enhanced NEXTflex® kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. The NEXTflex® rapid DNA-seq kit produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.

The NEXTflex® rapid DNA-seq 2.0 kit bundle offers all the advantages and reagents of the core kit, but with the additional benefit of including unique NEXTflex-HT barcodes for the number of reactions (8, 48, or 96 reaction size kits) in a single-use format. This allows the lab to tie all the necessary components of the library prep workflow to a single lot for ease of purchasing and tracking of consumables.

Figure 1: Libraries prepared with NEXTflex® rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K's kit using the same number of PCR cycles from a 250 ng and 1 ng input (A) and robust conversion rate (B).


Flexible Multiplexing Options

The NEXTflex® adapters are long, annealed adapters containing indexed sequences, which offer an improved multiplexing workflow and flexible experimental setups catered to individual needs. Our barcode portfolio encompass options for single or paired-end multiplex sequencing, and the NEXTflex® rapid DNA-seq kit 2.0 is compatible with the NEXTflex® DNA Barcodes and NEXTflex-96™ DNA Barcodes, NEXTflex® ChIP-Seq Barcodes, and NEXTflex-96™ ChIP-Seq BarcodesThe NEXTflex®rapid DNA-seq kit 2.0 reagents are also compatible with the NEXTflex® Unique Dual Index Barcodes  for up to 192 sample multiplexing.

The NEXTflex® rapid DNA-seq kit 2.0 bundles come provided with the NEXTflex-HT™ Barcodes.  

For more information about barcode options compatible with the NEXTflex® rapid DNA-seq kit 2.0, please contact bioo.ngs@perkinelmer.com.


Automation Compatibility

The NEXTflex® rapid DNA-seq kit 2.0 is compatible with multiple liquid handlers, including the PerkinElmer® Sciclone® G3 NGS workstation. For more information, or to obtain a program, contact bioo.ngs@perkinelmer.com.

For larger volume requirements, customization and bulk packaging is available. For increased flexibility, individual reagents (non-master mixed) are also available. Please contact bioo.ngs@perkinelmer.com for further information. 


Tech Tips - When to Size Select 

Size selection is a critical issue in NGS library preparation. Our blog post, Tech Tips – When to Size Select, addresses a number of factors to consider before determining a size selection strategy.


Kit Specs

The NEXTflex®  rapid DNA-seq 2.0 kits contain library prep reagents, including cleanup beads, to prepare 8, 48, or 96 genomic DNA samples for Illumina® sequencing. The bundled solution also includes the corresponding number of unique NEXTflex-HT barcodes per reaction. The shelf life of all reagents is 6 months when stored properly at -20°C. This kit is shipped on dry ice.

NEXTflex® Rapid DNA-Seq 2.0 Kit Library Prep Protocol

NEXTFLEX Rapid DNA-Seq Kit 2.0

Kit Contents

NEXTflex® End Repair & Adenylation Buffer Mix 2.0
NEXTflex® End Repair & Adenylation Enzyme Mix 2.0
NEXTflex® Ligase Buffer Mix 2.0
NEXTflex® Ligase Enzyme 2.0
NEXTflex® PCR Master Mix 2.0
NEXTflex® Primer Mix 2.0
NEXTflex® Cleanup Beads 2.0
Nuclease-free Water
Resuspension Buffer

 

Required Materials not Provided

1 ng - 1 µg of fragmented genomic DNA in up to 32 µL nuclease-free water.
NEXTflex® barcodes if purchasing a non-bundled kit (includes NEXTflex-HT barcodes)
Ethanol 100% (room temperature)
Ethanol 80% (room temperature)
Covaris System (S2, E210) or other method for DNA fragmentation
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Thermo Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (Bio-Rad, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher Scientific, Cat # AM10027) or similar
Heat block
Thermocycler 
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes
Vortex

Additional Data between NEXTflex® Rapid DNA-Seq 2.0 and Competitor K's Kit Libraries

 

Figure 1: Libraries prepared with NEXTflex® Rapid DNA-seq 2.0 shows less GC bias than libraries prepared with Competitor K's kit.

 

Figure 2: Libraries prepared with NEXTflex® Rapid DNA-seq 2.0 shows higher genome coverage than libraries prepared with Competitor K's kit.

What tools you should use to analyze your DNA-Seq data?

Read our blog post, Analysis Recommendations for DNA-Seq, to find out what tools we recommend to perform secondary and tertiary analysis.