NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for Ion Sequencing of FFPE DNA

Validated BRCA1/2 panel for use with DNA isolated from FFPE samples

Catalog# Product Name Quantity US List Price
4041-01
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible (Barcodes 1 - 8)
8 rxns $696 Buy Now
4041-02
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible (Barcodes 1 - 20)
20 rxns $1,613 Buy Now
4041-03
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible (Barcodes 21 - 40)
20 rxns $1,613 Buy Now
4041-04
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible (Barcodes 41 - 60)
20 rxns $1,613 Buy Now

NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible
  • Complete BRCA1 and BRCA2 coverage
  • 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples
  • Simple Ion Torrent targeted library prep - 4 hour protocol
  • For detection of somatic mutations, up to 20 samples can be multiplexed at 2000x coverage on the Ion Proton 
  • Complete solution for targeted sequencing, including gene specific primers, library prep reagents, cleanup beads and up to 60 unique barcodes
  • Low input - Only 40 ng DNA isolated from FFPE samples required

For Research Use Only. Not for use in diagnostic procedures.

Complete BRCA1 and BRCA2 Coverage of FFPE DNA for Sequencing on an Ion Torrent Platform

The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci, using 261 sequence validated PCR primer pairs. This kit requires only 40 ng of DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE protocol can be easily automated, further simplifying workflow.

NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE for Ion Torrent


NEXTflex™ BRCA1 & BRCA2 Amplicon Panel for FFPE Ion Torrent Platforms

Figure 1. Performance of 261 amplicons from NEXTflex™ BRCA1 & BRCA2 Amplicon Panel for FFPE (Ion Torrent Compatible) tested on FFPE sample

Kit Specs

The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE contains enough material to prepare 8 or 20 libraries for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

 

NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible Protocol

NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE Ion Torrent-Compatible Workflow

NEXTflex BRCA1/2 Amplicon Panel for Ion Platform Contents

NEXTflex™ BRCA Amplicon FFPE Ion Primer Mix 1 - 4
NEXTflex™ Hot Start PCR I Master Mix
NEXTflex™ End Repair Buffer Mix
NEXTflex™ End Repair Enzyme Mix
NEXTflex™ Ligation Mix
NEXTflex™ DNA P1 Adapter
NEXTflex™ DNA Barcoded Adapters
NEXTflex™ Primer Mix
NEXTflex™ PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex™ Cleanup Beads

 

Required Materials Not Provided

40-200 ng of DNA isolated from FFPE samples in up to 34 µL nuclease-free water
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
Thermocycler
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex