NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel for Illumina® Sequencing

Validated BRCA1/2 panel with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4221-01
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (8 Barcodes)
8 rxns $696 Buy Now
NOVA-4221-02
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (48 Barcodes)
48 rxns $3,870 Buy Now
NOVA-4221-03
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 1- 96)
96 rxns $7,129 Buy Now
NOVA-4221-04
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 97 - 192)
96 rxns $7,129 Buy Now
NOVA-4221-05
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 193 -288)
96 rxns $7,129 Buy Now
NOVA-4221-06
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 289 - 384) 96 rxns
$7,129 Buy Now

NEXTflex™ BRCA1 & BRCA2 XP Amplicon Panel Illumina-Compatible
  • Complete BRCA1 and BRCA2 coverage
  • 100% uniformity of all targeted coding exons and exon-intron boundaries
  • For detection of germline mutations multiplex up to 384 samples at 100x coverage and for detection of somatic mutations multiplex 14 samples at 2000x coverage on a single Illumina® 2x300 MiSeq lane
  • Complete solution for targeted sequencing including gene specific primers, PCR Master Mix, clean-up beads and up to 384 barcodes
  • Low input - Only 20 ng DNA required from fresh or frozen tissue or blood

For Research Use Only. Not for use in diagnostic procedures.

Complete BRCA1 and BRCA2 Coverage for Illumina Sequencing

The NEXTflex® BRCA1 & BRCA2 XP Amplicon Panel allows you to quickly target and sequence all exons of the BRCA1 and BRCA2 loci. This kit contains primer pairs and reagents needed to amplify the coding regions of BRCA1 and BRCA2 and prepare libraries for Illumina® sequencing. 

NEXTflex BRCA1/2 Amplicon Panels for Illumina Data


A Complete Solution for Targeted BRCA1/2 Sequencing

The NEXTflex BRCA1 & BRCA2 XP Amplicon Panel is a complete, validated solution that targets all exons in BRCA1 and BRCA2 loci using 131 PCR primer pairs. This kit requires only 20 ng of DNA from fresh or frozen samples. This kit is not designed for use with DNA isolated from FFPE or cfDNA samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 XP Amplicon Panel produces targeted libraries for Illumina sequencing® in 3 hours. Additionally, the NEXTflex BRCA1 & BRCA2 XP Amplicon Panel protocol can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex BRCA1 & BRCA2 XP Amplicon Panel contains enough material to prepare 8, 48 or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen tissue. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex BRCA1 and BRCA2 XP Amplicon Protocol for Illumina® Sequencing

NEXTflex BRCA1 & BRCA2 XP Amplicon Panel Workflow

NEXTflex BRCA1/2 XP Amplicon Panel Kit Contents

NEXTflex BRCA Amplicon Primer Mix 1
NEXTflex BRCA Amplicon Primer Mix 2
NEXTflex Hot Start PCR I Master Mix
NEXTflex End Repair Buffer Mix
NEXTflex End Repair Enzyme Mix
NEXTflex Ligation Mix
NEXTflex Amplicon DNA Adapter
NEXTflex PCR II Barcoded Primer Mix
NEXTflex PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex Cleanup Beads

Required Materials Not Provided

Two aliquots of 10-50 ng of high-quality genomic DNA in up to 34 µL nuclease-free water each
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Thermo Fisher®, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher®, Cat # AM10027) or similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Vortex
80% Ethanol, freshly prepared (room temperature)
1.5 mL nuclease-free microcentrifuge tubes

Related Kits:

For Use with DNA Isolated from Fresh or Frozen Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel – Illumina

NEXTflex® BRCA1 and BRCA2 Amplicon Panel – Ion Torrent

 

For Use with DNA Isolated from FFPE Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – Illumina