NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel for Illumina® Sequencing

Validated BRCA1/2 panel with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4221-01
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (8 Barcodes)
8 rxns $696 Buy Now
NOVA-4221-02
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (48 Barcodes)
48 rxns $3,870 Buy Now
NOVA-4221-03
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 1- 96)
96 rxns $7,129 Buy Now
NOVA-4221-04
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 97 - 192)
96 rxns $7,129 Buy Now
NOVA-4221-05
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 193 -288)
96 rxns $7,129 Buy Now
NOVA-4221-06
NEXTflex® BRCA1 and BRCA2 XP Amplicon Panel Illumina-Compatible (Barcodes 289 - 384) 96 rxns
$7,129 Buy Now

NEXTflex<sup>®</sup> BRCA1 & BRCA2 XP Amplicon Panel Illumina-Compatible
  • 100% coverage of BRCA1 and BRCA2 coding exons and flanking intron-exon boundaries
  • 100% uniformity of amplicons at 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA required from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the BRCA1 and BRCA2 Genes for Illumina® Sequencing

The NEXTflex® BRCA1 & BRCA2 XP Amplicon Panel allows you to quickly target and sequence all coding exons of the BRCA1 and BRCA2 loci. This kit contains primer pairs and reagents needed to amplify the coding regions of BRCA1 and BRCA2 from DNA isolated from fresh and frozen material, and prepare libraries for Illumina® sequencing. 

The NEXTflex® BRCA1 & BRCA2 XP Amplicon Panel has the capability of multiplexing up to 88 samples at 100x coverage for the detection of germline mutations and up to 4 samples at 2000x coverage for the detection of somatic mutations on a single Illumina® 2x300 Miseq® lane.


A Complete Solution for Targeted BRCA1 and BRCA2 Sequencing

The NEXTflex® BRCA1 & BRCA2 XP Amplicon Panel is a complete, validated solution that targets all coding exons in BRCA1 and BRCA2 loci using 132 PCR primer pairs. This kit requires only 20 ng of DNA from fresh or frozen samples. This kit is not designed for use with DNA isolated from FFPE or cfDNA samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® BRCA1 & BRCA2 XP Amplicon Panel contains enough material to prepare 8, 48 or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen tissue. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® BRCA1 and BRCA2 XP Amplicon Protocol for Illumina® Sequencing

NEXTflex BRCA1 & BRCA2 XP Amplicon Panel Workflow

NEXTflex® BRCA1/2 XP Amplicon Panel Kit Contents

NEXTflex® BRCA Amplicon Primer Mix 1
NEXTflex® BRCA Amplicon Primer Mix 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Stop Solution
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

Two aliquots of 10-50 ng of high-quality genomic DNA in up to 18 µL nuclease-free water each
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Thermo Fisher®, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher®, Cat # AM10027) or similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Vortex
80% Ethanol, freshly prepared (room temperature)
1.5 mL nuclease-free microcentrifuge tubes

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