NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing

Validated BRCA1/2 panel with library prep reagents and 60 barcodes for sequencing on any Ion platform

Catalog# Product Name Quantity US List Price
4040-01
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel Ion Torrent-Compatible (8 Barcodes)
8 rxns $696 Buy Now
4040-02
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel Ion Torrent-Compatible (1-20 Barcodes)
20 rxns $1,613 Buy Now
4040-03
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel Ion Torrent-Compatible (Barcodes 21 - 40)
20 rxns $1,613 Buy Now
4040-04
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel Ion Torrent-Compatible (Barcodes 41-60)
20 rxns $1,613 Buy Now

NEXTflex™ BRCA1 & BRCA2 Amplicon Panel Ion Torrent Compatible
  • Complete BRCA1 and BRCA2 coverage

  • 100% uniformity of all targeted coding exons and exon-intron boundaries
  • Simple Ion Torrent targeted library prep protocol - 4 hour protocol validated with low input samples
  • For detection of germline mutations multiplex up to 20 samples at 100x coverage on a single Ion 318TM Chip Kit v2 and for detection of somatic mutations multiplex up to 20 samples at 2000x coverage on Ion Proton 
  • Complete solution for targeted sequencing including gene specific primers, library prep reagents, and up to 20 barcodes
  • Low input - only 20 ng DNA required from fresh or frozen tissue or blood

Complete BRCA1 and BRCA2 Coverage for Sequencing on an Ion Platform

The NEXTflex BRCA1 & BRCA2 Amplicon Panel is a complete solution that targets all coding exons in BRCA1 and BRCA2 loci using 131 sequence validated PCR primer pairs.  This kit requires only 20 ng of DNA from fresh or frozen samples. This kit is not designed for DNA isolated from FFPE or cFDNA samples.The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. While competitor protocols typically require several hours to perform, the NEXTflex BRCA1 & BRCA2 Amplicon Panel makes it possible to produce targeted Ion Torrent-compatible libraries in only 4 hours. Additionally, the NEXTflex BRCA1 & BRCA2 Amplicon Panel protocol can be easily automated, further simplifying workflow.

NEXTflex BRCA1 & BRCA2 Amplicon Panel for Ion Torrent


Kit Specs

The NEXTflex™ BRCA1 & BRCA2 Amplicon Panel contains enough material to prepare 8, or 20 samples for Ion Torrent-compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

 

NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for Ion Torrent-based Sequencing Protocol

NEXTflex™ BRCA1 & BRCA2 Amplicon Panel Ion Torrent Compatible Flow Chart

NEXTflex BRCA1/2 Amplicon Panel for Ion Platform Contents

NEXTflex™ Amplicon BRCA Ion Primer Mix 1
NEXTflex™ Amplicon BRCA Ion Primer Mix 2
NEXTflex™ Hot Start PCR I Master Mix
NEXTflex™ End Repair Buffer Mix
NEXTflex™ End Repair Enzyme Mix
NEXTflex™ Ligation Mix
NEXTflex™ DNA P1 Adapter
NEXTflex™ DNA Barcoded Adapters
NEXTflex™ Primer Mix
NEXTflex™ PCR II Master Mix
Nuclease-free Water
Resuspension Buffer

 

Required Materials Not Provided

20-100 ng of high-quality genomic DNA in up to 34 µL nuclease-free water
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
Thermocycler
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex