NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE lllumina® Sequencing

Validated BRCA1/2 panel for use with DNA isolated from FFPE samples for Illumina® sequencing

Catalog# Product Name Quantity US List Price
4222-01
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (8 Barcodes)
8 rxns $696 Buy Now
4222-02
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (48 Barcodes)
48 rxns $3,870 Buy Now
4222-03
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (Barcodes 1 - 96)
96 rxns $7,129 Buy Now
4222-04
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (Barcodes 97 - 192)
96 rxns $7,129 Buy Now
4222-05
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (Barcodes 193 - 288)
96 rxns $7,129 Buy Now
4222-06
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$7,129 Buy Now

NEXTflex® BRCA1 & BRCA2 Amplicon Panel for FFPE Illumina-Compatible
  • Complete BRCA1 and BRCA2 coverage
  • 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples
  • Complete solution for targeted sequencing including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 40 ng DNA isolated from FFPE samples required

For Research Use Only. Not for use in diagnostic procedures.

Complete BRCA1 and BRCA2 Coverage for Illumina® Sequencing of FFPE DNA

The NEXTflex® BRCA1 & BRCA2 Amplicon Panel for FFPE allows you to quickly target and sequence all coding exons of the BRCA1 and BRCA2 loci. This kit contains primer pairs and reagents needed to amplify the coding regions of BRCA1 and BRCA2 from DNA isolated from FFPE material and prepare libraries for Illumina® sequencing. 

NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE Illumina-Compatible


A Complete Solution for Targeted BRCA1/2 Sequencing

The NEXTflex BRCA1 & BRCA2 Amplicon Panel for FFPE is a complete solution, validated with DNA isolated from FFPE samples, that targets all coding exons in BRCA1 and BRCA2 loci using 261 PCR primer pairs. This kit requires only 40 ng of DNA isolated from FFPE samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® BRCA1 & BRCA2 Amplicon Panel for FFPE contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from FFPE-fixed samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® BRCA1 and BRCA2 Amplicon Protocol for FFPE Illumina-Compatible

NEXTflex® BRCA1 & BRCA2 Amplicon Panels for FFPE Flow Chart

NEXTflex BRCA1/2 Amplicon Panel for FFPE Kit Contents

NEXTflex® BRCA Amplicon FFPE Primer Mix 1 - 4
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® End Repair Buffer Mix
NEXTflex® End Repair Enzyme Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix 1-8
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

40 - 200 ng of DNA isolated from FFPE samples (four 10 – 50 ng aliquots in up to 34 µL nuclease-free water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Ambion, Cat # AM10027) or similar
Thermocycler
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex