NEXTflex® CEBPA Amplicon Panel for  lllumina® Sequencing

Validated amplicon panel offering coverage of the CEBPA gene for use with DNA isolated from fresh or frozen samples for Illumina® sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4249-01
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $272 Buy Now
NOVA-4249-02
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $1,483 Buy Now
NOVA-4249-03
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $2,571 Buy Now
NOVA-4249-04
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $2,571 Buy Now
NOVA-4249-05
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $2,571
Buy Now
NOVA-4249-06
NEXTflex® CEBPA Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$2,571
Buy Now

NEXTflex CEBPA Amplicon Panel
  • 100% coverage of the CEBPA exon and flanking intron-exon boundaries 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of CEBPA for Illumina Sequencing

The NEXTflex® CEBPA Amplicon Panel allows you to quickly target and sequence the coding exon of the CEBPA locus. This kit contains primer pairs and reagents needed to amplify these coding regions of CEBPA from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex CEBPA Amplicon Panel, the panel has the capability of multiplexing up to 5,000 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

 

NEXTflex® CEBPA Amplicon Panel Data


A Complete Solution for Targeted CEBPA Sequencing

The NEXTflex CEBPA Amplicon Panel is a complete, validated solution that targets the coding exon in the CEBPA locus using 8 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® CEBPA Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® CEBPA Amplicon Panel Workflow

NEXTflex® CEBPA Amplicon Panel Protocol

NEXTflex CEBPA Amplicon Panel Kit Contents

NEXTflex® CEBPA Amplicon Primer Mix 1
NEXTflex® CEBPA Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 3
NEXTflex® PCR Enhancer 5
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 17 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific, Cat # AM10027) / or / similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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