NEXTflex™ Cardiovascular Disease Amplicon Panel

Validated panel for detection of the 22 hotspots for cardiovascular disease with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
4255-01
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $264 Buy Now
4255-02
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $1,440 Buy Now
4255-03
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $2,592 Buy Now
4255-04
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $2,592 Buy Now
4255-05
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $2,592
Buy Now
4255-06
NEXTflex™ Cardiovascular Disease Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$2,592
Buy Now

NEXTflex CVD Amplicon Panel
  • 100% coverage of 22 hotspots for cardiovascular disease 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Incorporates a single-tube format
  • Low input - Only 10 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of 22 Hotspots for Cardiovascular Disease

The NEXTflex™ Cardiovascular Disease Amplicon Panel contains primer pairs and reagents needed to amplify 22 hotspots for cardiovascular disease from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.


HUGO Gene Name Gene Name Chromosome reference SNP ID number
MTHFR Methylenetetrahydrofolate reductase chr1 rs1801133
MTHFR Methylenetetrahydrofolate reductase chr1 rs1801131
F5 Factor V chr1 rs1800595
F5 Factor V chr1 rs6025
F5 Factor V chr1 rs118203906
AGT Angiotensin chr1 rs699
APOB Apolipoprotein B chr2 rs5742904
AGTR1 Angiotensin II receptor type 1 chr3 rs5186
FGB Fibrinogen chr4 rs1800790
F13A1 Coagulation factor XIII chr6 rs5985
LTA Lymphotoxin alpha chr6 rs1041981
SERPINE1 Plasminogen activator inhibitor type 1 chr7 rs1799889
NOS3 Nitric oxide synthase 3 chr7 rs2070744
NOS3 Nitric oxide synthase 3 chr7 rs1799983
JAK-2 Janus kinase 2 chr9 rs10974944
F2 Prothrombin F2 chr11 rs1799963
ITGB3 Integrin beta-3 chr17 rs5918
APOE Apolipoprotein E chr19 rs429358
APOE Apolipoprotein E chr19 rs7412
PROCR Protein C receptor chr20 rs867186
PROCR Protein C receptor chr20 rs9574
CBS Cystathione β-synthase chr21 844IN68 (no rs)

Table 1. Hot spots covered by NEXTflexTM Cardiovascular Disease Amplicon Panel

 

While up to 384 barcodes are currently supplied with the NEXTflex Cardiovascular Disease Amplicon Panel, the panel has the capability of multiplexing up to 1,700 samples at 100x coverage and 90 samples at 2000x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.


A Complete Solution for Sequencing of Cardiovascular Disease Hotspots 

The NEXTflex Cardiovascular Disease Amplicon Panel is a complete, validated solution that targets 22 hotspots for cardiovascular disease using 20 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Cardiovascular Disease Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex™ Cardiovascular Disease Amplicon Panel

NEXTFLEX™ CVD Amplicon Panel Protocol

NEXTflex Cardiovascular Disease Amplicon Panel Kit Contents

NEXTflex™ CVD Amplicon Primer Mix
NEXTflex™ PCR Enhancer 2
NEXTflex™ Hot Start PCR I Master Mix
NEXTflex™ Ligation Mix
NEXTflex™ Amplicon DNA Adapter
NEXTflex™ PCR II Barcoded Primer Mix
NEXTflex™ PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex™ Cleanup Beads

Required Materials Not Provided

10 - 50 ng of extracted genomic DNA (in up to 27 μL nuclease-free water) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

Related Kits:

For Use with DNA Isolated from Fresh or Frozen Samples

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NEXTflex™ BRCA1 and BRCA2 Amplicon Panel - for Ion Torrent platforms

 

For Use with DNA Isolated from FFPE Samples

NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex™ TP53 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE – for Ion Torrent platforms


For Research Use Only. Not for use in diagnostic procedures.