NEXTflex® Congenital Hyperinsulinism Amplicon Panel

Validated panel for detection of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 genes with library prep reagents and 384 barcodes for Illumina® sequencing.

Catalog# Product Name Quantity US List Price
4245-01
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $616 Buy Now
4245-02
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,552 Buy Now
4245-03
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,720 Buy Now
4245-04
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,720 Buy Now
4245-05
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,720
Buy Now
4245-06
NEXTflex® Congenital Hyperinsulinism Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,720
Buy Now

NEXTflex® CHI Amplicon Panel
  • 100% coverage of ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 

    exons and flanking intron-exon boundaries 

  • 98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 Genes for Illumina® Sequencing

The NEXTflex® Congenital Hyperinsulinism Amplicon Panel allows you to quickly target and sequence 119 exons of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 loci and two pathological mutations in the SLC16A1 promoter and one pathological mutation in HADH intron 6 region. This kit contains primer pairs and reagents needed to amplify these coding regions of the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 genes and two pathological mutations in the SLC16A1 promoter and one pathological mutation in HADH intron 6 region from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Congenital Hyperinsulinism Amplicon Panel, the panel has the capability of multiplexing up to 180 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

NEXTflex® CHI Amplicon Panel Table


A Complete Solution for Targeted Sequencing

The NEXTflex Congenital Hyperinsulinism Amplicon Panel is a complete, validated solution that targets 119 exons in the ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 and UCP2 loci and two pathological mutations in the SLC16A1 promoter and one pathological mutation in HADH intron 6 region using 212 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Congenital Hyperinsulinism Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Congenital Hyperinsulinism Amplicon Panel

Congenital Hyperinsulinism Flow Chart

NEXTflex Congenital Hyperinsulinism Amplicon Panel Kit Contents

NEXTflex® CHI Amplicon Primer Mix 1
NEXTflex® CHI Amplicon Primer Mix 2
PCR Enhancer 1
PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 31.5 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

Related Kits:

For Use with DNA Isolated from Fresh or Frozen Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel – for Illumina® platforms
NEXTflex® BRCA1 and BRCA2 Plus-1 Amplicon Panel - for Illumina® platforms
NEXTflex® Cardiovascular Disease Amplicon Panel - for Illumina® platforms
NEXTflex® CEBPA Amplicon Panel – for Illumina® platforms
NEXTflex® Colorectal Cancer-1 Amplicon Panel - for Illumina® platforms
NEXTflex® Colorectal Cancer-2 Amplicon Panel for Illumina® platforms
NEXTflex® Congenital Adrenal Hyperplasia Amplicon Panel for Illumina® platforms
NEXTflex® Diabetes-3 Amplicon Panel - for Illumina® platforms
NEXTflex® Diabetes-4 Amplicon Panel - for Illumina® platforms
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel - for Illumina® platforms
NEXTflex® Epilepsy-2 Amplicon Panel - for Illumina® platforms
NEXTflex® Female Infertility Amplicon Panel for Illumina® platforms
NEXTflex® HBOC-1 Amplicon Panel for Illumina® platforms
NEXTflex® HBOC-3 Amplicon Panel - for Illumina® platforms 
NEXTflex® Lysosomal Storage Diseases Amplicon Panel - for Illumina® platforms
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms
NEXTflex® Marfan Syndrome Amplicon Panel - for Illumina® platforms
NEXTflex® Mediterranean Fever Amplicon Panel - for Illumina® platforms
NEXTflex® MODY-1 Amplicon Panel – for Illumina® platforms
NEXTflex® MODY-2 Amplicon Panel – for Illumina® platforms
NEXTflex® MODY-5 Amplicon Panel for lllumina® Platforms   
NEXTflex® Myeloid Amplicon Panel for lllumina® platforms
NEXTflex® Nephrotic Syndrome-1 Amplicon Panel - for Illumina® platforms
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel - for Illumina® platforms
NEXTflex® Neurofibromatosis Amplicon Panel - for Illumina® platforms
NEXTflex® Neuronal Ceroid Lipofuscinoses Amplicon Panel for lllumina® platforms
NEXTflex® Obesity-1 Amplicon Panel - for Illumina® platforms 
NEXTflex® Obesity-2 Amplicon Panel for Illumina® platforms