NEXTflex® Cystic Fibrosis Amplicon Panel

Validated panel for detection of the CFTR gene with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4231-01
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $386 Buy Now
NOVA-4231-02
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $2,163 Buy Now
NOVA-4231-03
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $4,005 Buy Now
NOVA-4231-04
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $4,005 Buy Now
NOVA-4231-05
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $4,005
Buy Now
NOVA-4231-06
NEXTflex® Cystic Fibrosis Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$4,005
Buy Now

NEXTflex<sup>®</sup> Cystic Fibrosis Amplicon Panel for Illumina® Platforms
  • 100% coverage of CFTR exons and flanking intron-exon boundaries 
  • 98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the CFTR Gene for Illumina® Sequencing

The NEXTflex® Cystic Fibrosis Amplicon Panel allows you to quickly target and sequence 28 exons of the CFTR locus. This kit contains primer pairs and reagents needed to amplify these coding regions of the CFTR gene from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Cystic Fibrosis Amplicon Panel, the panel has the capability of multiplexing up to 600 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

Cystic Fibrosis Data


A Complete Solution for Targeted CFTR Sequencing

The NEXTflex Cystic Fibrosis Amplicon Panel is a complete, validated solution that targets 28 exons in the CFTR locus using 61 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Cystic Fibrosis Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Cystic Fibrosis Amplicon Panel

Cystic Fibrosis Flow Chart

NEXTflex Cystic Fibrosis Amplicon Panel Kit Contents

NEXTflex® CFTR Amplicon Primer Mix 1
NEXTflex® CFTR Amplicon Primer Mix 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 32 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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