NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel

Validated panel for detection of the DMD gene with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4256-01
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $824 Buy Now
NOVA-4256-02
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $4,800 Buy Now
NOVA-4256-03
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $9,312 Buy Now
NOVA-4256-04
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $9,312 Buy Now
NOVA-4256-05
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $9,312
Buy Now
NOVA-4256-06
NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$9,312
Buy Now

NEXTflex Duchenne Muscular Dystrophy Amplicon Panel
  • 100% coverage of the DMD exons and flanking intron-exon boundaries 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the DMD Gene for Illumina® Sequencing

The NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel allows you to quickly target and sequence 86 exons of the DMD locus. This kit contains primer pairs and reagents needed to amplify these coding regions of the DMD locus from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTflex Duchenne Muscular Dystrophy Amplicon Panel has the capability of multiplexing up to 240 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. 


A Complete Solution for Targeted DMD Sequencing

The NEXTflex Duchenne Muscular Dystrophy Amplicon Panel is a complete, validated solution that targets 86 exons in the DMD locus using 167 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Duchenne Muscular Dystrophy Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Duchenne Muscular Dystrophy Amplicon Panel

Duchenne Muscular Dystrophy Flow Chart

NEXTflex Duchenne Muscular Dystrophy Amplicon Panel Kit Contents

NEXTflex® DMD Amplicon Primer Mix 1
NEXTflex® DMD Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 4
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 31.2 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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