NEXTflex® Epilepsy-2 Amplicon Panel

Validated panel for detection of the SCN2A and SCN9A genes with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
4236-01
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $616 Buy Now
4236-02
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,552 Buy Now
4236-03
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,720 Buy Now
4236-04
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,720 Buy Now
4236-05
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,720
Buy Now
4236-06
NEXTflex® Epilepsy-2 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,720
Buy Now

NEXTflex Epilepsy-2 Amplicon Panel
  • 100% coverage of SCN2A and SCN9A exons and flanking intron-exon boundaries 
  • 98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the SCN2A and SCN9A Genes for Illumina® Sequencing

The NEXTflex® Epilepsy-2 Amplicon Panel allows you to quickly target and sequence 54 exons of the SCN2A and SCN9A loci. This kit contains primer pairs and reagents needed to amplify these coding regions of the SCN2A and SCN9A genes from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Epilepsy-2 Amplicon Panel, the panel has the capability of multiplexing up to 300 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.


A Complete Solution for Targeted SCN2A and SCN9A Sequencing

The NEXTflex Epilepsy-2 Amplicon Panel is a complete, validated solution that targets 54 exons in the SCN2A and SCN9A loci using 117 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Epilepsy-2 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Epilepsy-2 Amplicon Panel

Epilepsy-2 Flow Chart

NEXTflex Epilepsy-2 Amplicon Panel Kit Contents

NEXTflex® Epilepsy-2 Amplicon Primer Mix 1
NEXTflex® Epilepsy-2 Amplicon Primer Mix 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 34 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Use with DNA Isolated from Fresh or Frozen Samples

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For Use with DNA Isolated from FFPE Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex® TP53 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Ion Torrent platforms


For Research Use Only. Not for use in diagnostic procedures.