NEXTflex® HBOC-3 Amplicon Panel for lllumina Sequencing

Validated amplicon panel offering coverage of the PALB2, BARD1 and TP53 genes for use with DNA isolated from fresh or frozen samples for Illumina sequencing

Catalog# Product Name Quantity US List Price
4240-01
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $608 Buy Now
4240-02
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,502 Buy Now
4240-03
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,674 Buy Now
4240-04
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,674 Buy Now
4240-05
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,674 Buy Now
4240-06
NEXTflex® HBOC-3 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,674 Buy Now

NEXTflex HBOC-3 Amplicon Panel Illumina-Compatible
  • 100% coverage of PALB2, BARD1 and TP53 exons and flanking intron-exon boundaries 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of PALB2, BARD1 and TP53 Genes for Illumina Sequencing

The NEXTflex® HBOC-3 Amplicon Panel allows you to quickly target and sequence 38 exons of the PALB2, BARD1 and TP53 loci, vital genes for research of Hereditary Breast and Ovarian Cancer Syndrome (HBOC). This kit contains primer pairs and reagents needed to amplify these coding regions of PALB2, BARD1 and TP53 from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex HBOC-3 Amplicon Panel, the panel has the capability of multiplexing up to 700 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

 

NEXTflex HBOC-3 Amplicon Panel


A Complete Solution for Targeted PALB2, BARD1 and TP53 Sequencing

The NEXTflex HBOC-3 Amplicon Panel is a complete, validated solution that targets 38 exons in PALB2, BARD1 and TP53 loci using 73 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® HBOC-3 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® HBOC-3 Amplicon Panel Workflow

NEXTflex® HBOC-3 Amplicon Panel Protocol

NEXTflex HBOC-3 Amplicon Panel Kit Contents

NEXTflex® HBOC-3 Amplicon Primer Mix 1
NEXTflex® HBOC-3 Amplicon Primer Mix 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 34 μL nuclease-free water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher Scientific, Cat # AM10027) or similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex