NEXTflex-HT™ Barcode Adapter (25 µM)
NEXTflex™ Primer Mix (12.5 µM)
Sequences of NEXTflex-HT Barcode Indexes - Excel / PDF
What is the concentration of the NEXTflex-HT Barcodes?
The concentration of the NEXTflex-HT Barcodes is 25 µM.
Are these barcodes compatible with both paired-end and single-read Illumina sequencing?
Yes, the NEXTflex-HT Barcodes can be used with both SR and PE sequencing.
Do the NEXTflex-HT Barcodes Kits contain PCR primers?
Yes, these barcodes are supplied with PCR primers.
At what concentration are the NEXTflex PCR primers supplied?
The NEXTflex PCR Primers concentration is 12.5 µM.
Can I order more NEXTflex PCR primers alone?
Yes, the NEXTflex PCR Primers are available in sets of 48, 96, 192, 384 or 768 reactions. Contact firstname.lastname@example.org for more information.
How is the quality of the NEXTflex-HT Barcodes assured?
Each lot of the NEXTflex barcodes is sequence verified on an Illumina MiSeq.
What type of purification is used when the NEXTflex DNA Barcodes are synthesized?
In order to ensure the highest quality sequencing data, the NEXTflex-HT Barcodes are HPLC-purified, minimizing the risk of mispriming events due to truncations and other data quality-compromising errors.
Are the NEXTflex-HT Barcodes methylated?
No they are not methylated; therefore they are not compatible with bisulfite-seq applications. However, they are compatible with all other DNA-Seq applications including MeDIP and MeCAP sequencing. The NEXTflex Bisulfite-Seq Barcodes are provided for library prep requiring methylated adapters.