NEXTflex® Lysosomal Storage Diseases Amplicon Panel

Validated panel for detection of the SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS genes with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4259-01
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $544 Buy Now
NOVA-4259-02
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,120 Buy Now
NOVA-4259-03
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $5,952 Buy Now
NOVA-4259-04
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $5,952 Buy Now
NOVA-4259-05
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $5,952
Buy Now
NOVA-4259-06
NEXTflex® Lysosomal Storage Diseases Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$5,952
Buy Now

NEXTflex® LSD Amplicon Panel
  • 100% coverage of SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS exons and flanking intron-exon boundaries 
  • 99% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS Genes for Illumina® Sequencing

The NEXTflex® Lysosomal Storage Diseases Amplicon Panel allows you to quickly target and sequence 136 exons of the SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS loci. This kit contains primer pairs and reagents needed to amplify these coding regions of the SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS genes from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTflex Lysosomal Storage Diseases Amplicon Panel has the capability of multiplexing up to 75 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.


A Complete Solution for Targeted SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS Sequencing

 

The NEXTflex Lysosomal Storage Diseases Amplicon Panel is a complete, validated solution that targets 136 exons in the SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA and IDS  loci using 258 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Lysosomal Storage Diseases Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Lysosomal Storage Diseases Amplicon Panel

Lysosomal Storage Diseases Flow Chart

NEXTflex Lysosomal Storage Diseases Amplicon Panel Kit Contents

NEXTflex® LSD Amplicon Primer Mix 1
NEXTflex® LSD Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 26 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Research Use Only. Not for use in diagnostic procedures.