NEXTflex® MODY-1 Amplicon Panel

Validated panel for detection of GCK and HNF1A genes with library prep reagents and barcodes for Illumina® sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4232-01
NEXTflex® MODY-1 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $386 Buy Now
NOVA-4232-02
NEXTflex® MODY-1 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $2,163 Buy Now
NOVA-4232-03
NEXTflex® MODY-1 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $4,005 Buy Now
* NEXTflex® amplicon panels containing barcodes 97 to 384 are still available as a custom order request.

NEXTflex™ MODY-1 Amplicon Panel for Illumina® Platforms
  • 100% coverage of GCK and HNF1A exons and flanking intron-exon boundaries 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available upon request
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of GCK and HNF1A Genes for Illumina® Sequencing

The NEXTflex® MODY-1 Amplicon Panel allows you to quickly target and sequence 22 exons of the GCK and HNF1A loci. This kit contains primer pairs and reagents needed to amplify these coding regions of GCK and HNF1A from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTflex® MODY-1 Amplicon Panel has the capability of multiplexing up to 505 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x250 MiSeq® lane. 

 


A Complete Solution for Targeted GCK and HNF1A Sequencing

The NEXTflex® MODY-1 Amplicon Panel is a complete, validated solution that targets 22 exons in GCK and HNF1A loci using 42 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® MODY-1 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® MODY-1 Amplicon Panel

Periodic Fever-2 Flow Chart

NEXTflex MODY-1 Amplicon Panel Kit Contents

NEXTflex® MODY-1 Amplicon Primer Mix 1
NEXTflex® MODY-1 Amplicon Primer Mix 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Stop Solution
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 20.4 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Use with DNA Isolated from Fresh or Frozen Samples

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