NEXTflex® Diabetes-4 Amplicon Panel for lllumina Sequencing

Validated amplicon panel offering coverage of the PAX4, INS and BLK genes for use with DNA isolated from fresh or frozen samples for Illumina sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4238-01
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $494 Buy Now
NOVA-4238-02
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $2,818 Buy Now
NOVA-4238-03
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $5,340 Buy Now
NOVA-4238-04
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $5,340 Buy Now
NOVA-4238-05
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $5,340
Buy Now
NOVA-4238-06
NEXTflex® Diabetes-4 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$5,340
Buy Now

NEXTflex Diabetes-4 Amplicon Panel Illumina-Compatible
  • 100% coverage of PAX4, INS and BLK exons and flanking intron-exon boundaries 
  • 100% uniformity of amplicons on 0.2x coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of PAX4, INS and BLK Genes for Illumina Sequencing

The NEXTflex® Diabetes-4 Amplicon Panel allows you to quickly target and sequence 26 exons of the PAX4, INS and BLK loci. This kit contains primer pairs and reagents needed to amplify these coding regions of PAX4, INS and BLK from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Diabetes-4 Amplicon Panel, the panel has the capability of multiplexing up to 700 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

 

NEXTflex Diabetes-4 Amplicon Panel


A Complete Solution for Targeted PAX4, INS and BLK Sequencing

The NEXTflex Diabetes-4 Amplicon Panel is a complete, validated solution that targets 26 exons in PAX4, INS and BLK loci using 38 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® Diabetes-4 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Diabetes-4 Amplicon Panel Workflow

NEXTflex™ Diabetes-4 Amplicon Panel Protocol

NEXTflex Diabetes-4 Amplicon Panel Kit Contents

NEXTflex® Diabetes-4 Amplicon Primer Mix 1
NEXTflex® Diabetes-4 Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 30 μL nuclease-free water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher Scientific, Cat # AM10027) or similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex

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