- 100% coverage of GCK, HNF1A, HNF1B, and HNF4A exons and flanking intron-exon boundaries
- 100% uniformity of amplicons at 0.1x mean coverage
- 99% uniformity of amplicons at 0.2x mean coverage
- 384 unique barcodes available upon request
- Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and barcodes
- Low input - Only 20 ng DNA isolated from fresh or frozen samples required
For research use only. Not for use in diagnostic procedures.
Coverage of GCK, HNF1A, HNF1B, and HNF4A Genes for Illumina Sequencing
The NEXTflex® MODY-5 Amplicon Panel allows you to quickly target and sequence 44 exons of the GCK, HNF1A, HNF1B, and HNF4A loci, vital genes for research of Maturity Onset Diabetes of the Young (MODY). This kit contains primer pairs and reagents needed to amplify these coding regions of GCK, HNF1A, HNF1B, and HNF4A from DNA isolated from fresh or frozen material and prepare libraries for Illumina sequencing.
The NEXTflex® MODY-5 Amplicon Panel has the capability of multiplexing up to 150 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.
A Complete Solution for Targeted GCK, HNF1A, HNF1B, and HNF4A Sequencing
The NEXTflex® MODY-5 Amplicon Panel is a complete, validated solution that targets 44 exons in GCK, HNF1A, HNF1B, and HNF4A loci using 78 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. This convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. The kit can be easily automated on liquid handlers, further simplifying workflow.
The NEXTflex® MODY-5 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
NEXTflex MODY-5 Amplicon Panel Kit Contents
NEXTflex® MODY-5 Amplicon Primer Mix 1
NEXTflex® MODY-5 Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (B)
NEXTflex® Stop Solution
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
NEXTflex® Cleanup Beads
Required Materials Not Provided
20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 15.9 μL nuclease-free water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
1.5 mL nuclease-free microcentrifuge tubes