NEXTflex® Male Infertility Amplicon Panel for lllumina® Sequencing Platforms

Validated amplicon panel offering coverage of the AR, CATSPER1, CFTR, FSHR, and LHCGR genes for use with DNA isolated from fresh or frozen samples for Illumina® sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4262-01
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $696 Buy Now
NOVA-4262-02
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,870 Buy Now
NOVA-4262-03
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $7,129 Buy Now
NOVA-4262-04
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $7,129 Buy Now
NOVA-4262-05
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $7,129 Buy Now
NOVA-4262-06
NEXTflex® Male Infertility Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$7,129 Buy Now

 

NEXTFLEX® Female Infertility Amplicon Panel
  • 100% coverage of AR, CATSPER1, CFTR, FSHR, and LHCGR exons and flanking intron-exon boundaries 
  • 98% uniformity of amplicons at 0.2x mean coverage
  • 99% uniformity of amplicons at 0.1x mean coverage
  • 100% uniformity of amplicons at 0.05x mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of AR, CATSPER1, CFTR, FSHR, and LHCGR Genes for Illumina Sequencing

The NEXTflex® Male Infertility Amplicon Panel allows you to quickly target and sequence 75 exons of the AR, CATSPER1, CFTR, FSHR, and LHCGR loci, vital genes for research of male infertility. This kit contains primer pairs and reagents needed to amplify these coding regions of AR, CATSPER1, CFTR, FSHR,  LHCGR from DNA isolated from fresh or frozen material and prepare libraries for Illumina® sequencing.

The NEXTflex® Male Infertility Amplicon Panel has the capability to multiplex up to 65 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more samples, contact Bioo.NGS@perkinelmer.com to request a quote for a panel with additional barcodes.

 

 


A Complete Solution for Targeted AR, CATSPER1, CFTR, FSHR, and LHCGR Sequencing

The NEXTflex® Male Infertility Amplicon Panel is a complete, validated solution that targets 75 exons in AR, CATSPER1, CFTR, FSHR, and LHCGR loci using 168 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® Male Infertility Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Male Infertility Amplicon Panel Workflow

NEXTflex® Male Infertility Amplicon Panel Protocol

NEXTflex Male Infertility Amplicon Panel Kit Contents

NEXTflex® Male Infertility Amplicon Primer Mix 1
NEXTflex® Male Infertility Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 3
NEXTflex® PCR Enhancer 4
NEXTflex® Hot Start PCR I Master Mix (B)
NEXTflex® Stop Solution
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 16.3 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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