NEXTflex® Mediterranean Fever Amplicon Panel for lllumina Sequencing

Validated panel for detection of mutations in the MEFV gene in DNA isolated from fresh or frozen samples for Illumina sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4237-01
NEXTflex® Mediterranean Fever  Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $386 Buy Now
NOVA-4237-02
NEXTflex® Mediterranean Fever  Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $2,163 Buy Now
NOVA-4237-03
NEXTflex® Mediterranean Fever Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $4,005 Buy Now
NOVA-4237-04
NEXTflex® Mediterranean Fever Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $4,005 Buy Now
NOVA-4237-05
NEXTflex® Mediterranean Fever Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $4,005 Buy Now
NOVA-4237-06
NEXTflex® Mediterranean Fever Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$4,005 Buy Now

NEXTflex MEFV Amplicon Panel
  • 100% coverage of MEFV and flanking intron-exon boundaries (4 kb total)
  • 100% uniformity of amplicons at 0.2X mean coverage
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads and up to 384 barcodes
  • Low input - Only 20 ng DNA required

For research use only. Not for use in diagnostic procedures.

MEFV Coverage for Illumina Sequencing

The NEXTflex® Mediterranean Fever Amplicon Panel allows you to quickly target and sequence 10 canonical exons of the MEFV loci. This kit contains primer pairs and reagents needed to amplify these coding regions of MEFV from DNA isolated from fresh or frozen samples and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Mediterranean Fever Amplicon Panel, the panel has the capability of multiplexing up to 1600 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

 

NEXTflex MEFV Amplicon Panel Data


A Complete Solution for Targeted MEFV Sequencing

The NEXTflex Mediterranean Fever Amplicon Panel is a complete, validated solution that targets 10 canonical exons in MEFV loci using 24 PCR primer pairs. This kit requires only 20 ng of genomic DNA. This kit can be easily automated on liquid handlers.


Kit Specs

The NEXTflex Mediterranean Fever Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Mediterranean Fever  Amplicon Panel Illumina-Compatible Workflow

NEXTflex™ MEFV Amplicon Panel Flowchart

NEXTflex Mediterranean Fever Amplicon Panel Kit Contents

NEXTflex® MEFV Amplicon Primer Mix 1
NEXTflex® MEFV Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 26 μL nuclease-free water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex

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