NEXTflex® Myeloid Amplicon Panel

Validated panel for detection of 105 selected exons from 21 Myeloid related genes (see table below) with library prep reagents and 384 barcodes for Illumina® sequencing.

Catalog# Product Name Quantity US List Price
NOVA 4260-01
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $704 Buy Now
NOVA 4260-02
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $4,032 Buy Now
NOVA 4260-03
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $7,680 Buy Now
NOVA 4260-04
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $7,680 Buy Now
NOVA 4260-05
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $7,680
Buy Now
NOVA 4260-06
NEXTflex® Myeloid Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$7,680
Buy Now

NEXTFLEX® Myeloid Amplicon Panel
  • 100% coverage of 105 selected exons from 21 different genes and flanking intron-exon boundaries 
  • 95% uniformity of amplicons at 0.2x mean coverage; 99% of amplicons detected at 0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 40 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of the selected Genes for Illumina® Sequencing Platforms

The NEXTflex® Myeloid Amplicon Panel allows you to quickly target and sequence 105 selected exons from 21 different genes loci. This kit contains primer pairs and reagents needed to amplify these coding regions from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Myeloid Amplicon Panel, the panel has the capability of multiplexing up to 4 samples at 2000x coverage for detection of somatic mutations on a single Illumina® 2x300 MiSeq® lane.


Gene Covered Region
ASXL1 Exon 12
CALR Exon 9
CEBPA All CDS*
CSF3R Exon 14, 17
DNMT3A All CDS*
EZH2 All CDS*
FLT3 Exon 14-15, 20
IDH1 Exon 4
IDH2 Exon 4
JAK2 Exon 12, 14
JAK3 Exon 13
KIT Exon 2, 8-11, 13, 17
MPL Exon 10
NPM1 Exon 11
RUNX1 All CDS*
SETBP1 Exon 4 (hotspot)
SF3B1 Exon 13-16
SRSF2 Exon 2
TET2 Exon 3-11
TP53 Exon 2-11
U2AF1 Exon 2, 5, 6

* CDS = Coding Sequences

A Complete Solution for Targeted Sequencing

The NEXTflex Myeloid Amplicon Panel is a complete, validated solution that targets 105 selected exons from 21 different genes loci using 270 PCR primer pairs. This kit requires only 40 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Myeloid Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Myeloid Amplicon Panel

NEXTflex® Myeloid Amplicon Panel Protocol

NEXTflex Myeloid Amplicon Panel Kit Contents

NEXTflex®  Myeloid Amplicon Primer Mix 1 and 2
NEXTflex®  Myeloid Amplicon Primer Mix 3 and 4
NEXTflex®  Hot Start PCR I Master Mix
NEXTflex®  Hot Start PCR I Master Mix (A)
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® PCR Enhancer 5
NEXTflex® Stop Solution
NEXTflex®  Ligation Mix
NEXTflex®  Amplicon DNA Adapter
NEXTflex®  PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

40 - 200 ng of extracted genomic DNA (four 10 - 50 ng aliquots in up to 19.8 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Use with DNA Isolated from FFPE Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex® TP53 Amplicon Panel for FFPE – for Illumina® platforms

 

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Ion Torrent platforms