NEXTflex® Nephrotic Syndrome-2 Amplicon Panel

Validated panel for detection of the ARHGDIA, DGKE, LAMB2 and PLCE1 genes with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4254-01
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $592 Buy Now
NOVA-4254-02
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,408 Buy Now
NOVA-4254-03
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,528 Buy Now
NOVA-4254-04
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,528 Buy Now
NOVA-4254-05
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,528
Buy Now
NOVA-4254-06
NEXTflex® Nephrotic Syndrome-2 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,528
Buy Now

NEXTflex® Nephrotic Syndrome-2 Amplicon Panel
  • 100% coverage of ARHGDIA, DGKE, LAMB2 and PLCE1 exons and flanking intron-exon boundaries 
  • 99% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For Research Use Only. Not for use in diagnostic procedures.

Coverage of the ARHGDIA, DGKE, LAMB2 and PLCE1 Genes for Illumina® Sequencing

The NEXTflex® Nephrotic Syndrome-2 Amplicon Panel allows you to quickly target and sequence 81 exons of the ARHGDIA, DGKE, LAMB2 and PLCE1 loci. This kit contains primer pairs and reagents needed to amplify these coding regions of the ARHGDIA, DGKE, LAMB2 and PLCE1 genes from DNA isolated from fresh or frozen material, and prepare libraries for Illumina® sequencing.

The NEXTflex Nephrotic Syndrome-2 Amplicon Panel has the capability of multiplexing up to 275 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane.


A Complete Solution for Targeted ARHGDIA, DGKE, LAMB2 and PLCE1 Sequencing

The NEXTflex Nephrotic Syndrome-2 Amplicon Panel is a complete, validated solution that targets 81 exons in the ARHGDIA, DGKE, LAMB2 and PLCE1 loci using 140 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Nephrotic Syndrome-2 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Nephrotic Syndrome-2 Amplicon Panel

Nephrotic Syndrome-2 Flow Chart

NEXTflex Nephrotic Syndrome-2 Amplicon Panel Kit Contents

NEXTflex® Nephrotic Syndrome-2 Amplicon Primer Mix 1
NEXTflex® Nephrotic Syndrome-2 Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 26 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research™, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Use with DNA Isolated from Fresh or Frozen Samples

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For Use with DNA Isolated from FFPE Samples

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Illumina® platforms
NEXTflex® TP53 Amplicon Panel for FFPE – for Illumina® platforms

NEXTflex® BRCA1 and BRCA2 Amplicon Panel for FFPE – for Ion Torrent™ platforms

 

For Research Use Only. Not for use in diagnostic procedures.