NEXTflex® Obesity-1 Amplicon Panel for lllumina Sequencing

Validated panel for detection of mutations in the LEP, LEPR, POMC, and MC4R genes in DNA isolated from fresh or frozen samples for Illumina sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4241-01
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $608 Buy Now
NOVA-4241-02
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,502 Buy Now
NOVA-4241-03
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,674 Buy Now
NOVA-4241-04
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,674 Buy Now
NOVA-4241-05
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,674 Buy Now
NOVA-4241-06
NEXTflex® Obesity-1 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,674 Buy Now

NEXTflex Obesity-1 Amplicon Panel
  • 100% coverage of LEP, LEPR, POMC, and MC4R exons and flanking intron-exon boundaries (9.5 kb total)
  • 100% uniformity amplicons detected at 0.2X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA required

For research use only. Not for use in diagnostic procedures.

Coverage of LEP, LEPR, POMC, and MC4R genes for Illumina Sequencing

The NEXTflex® Obesity-1 Amplicon Panel allows rapid targeting and sequencing of the 26 coding exons of the LEP, LEPR, POMC, and MC4R loci. This kit contains primer pairs and reagents needed to amplify the coding regions of LEP, LEPR, POMC, and MC4R from DNA isolated from fresh or frozen samples and prepare libraries for Illumina sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Obesity-1 Amplicon Panel, the panel has the capability of multiplexing up to 700 samples at 100x coverage for detection of germline mutations on a single Illumina 2x300 MiSeq lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

 

NEXTflex Obesity-1 Amplicon Panel Data


A Complete Solution for Targeted Sequencing

The NEXTflex Obesity-1 Amplicon Panel is a complete, validated solution that targets 26 coding exons in LEP, LEPR, POMC, and MC4R loci using 60 PCR primer pairs. This kit requires only 20 ng of genomic DNA. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex Obesity-1 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Obesity-1 Amplicon Protocol Illumina-Compatible

NEXTflex™ Obesity-1 Amplicon Panel Flowchart

NEXTflex Obesity-1 Amplicon Panel Kit Contents

NEXTflex® Obesity-1 Amplicon Primer Mix 1
NEXTflex® Obesity-1 Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

 

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 31.5 μL nucleasefree water each)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) or similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) or similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
Thermocycler
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex

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