NEXTflex^® Obesity-2 Amplicon Panel

• 100% coverage of KSR2, SH2B1 and SIM1 exons and flanking intron-exon boundaries 
• 97% uniformity of amplicons on 0.2x coverage; 99% of amplicons detected at >0.1X mean coverage
• 384 unique barcodes available
• Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
• Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of the KSR2, SH2B1 and SIM1 Genes for Illumina^® Sequencing

The NEXTflex^® Obesity-2 Amplicon Panel allows you to quickly target and sequence 41 exons of the KSR2, SH2B1 and SIM1 loci. This kit contains primer pairs and reagents needed to amplify these coding regions of the KSR2, SH2B1 and SIM1 genes from DNA isolated from fresh or frozen material, and prepare libraries for Illumina^® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Obesity-2 Amplicon Panel, the panel has the capability of multiplexing up to 500 samples at 100x coverage for detection of germline mutations on a single Illumina^® 2x300 MiSeq^® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

A Complete Solution for Targeted KSR2, SH2B1 and SIM1 Sequencing

The NEXTflex Obesity-2 Amplicon Panel is a complete, validated solution that targets 41 exons in the KSR2, SH2B1 and SIM1 loci using 71 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.

Kit Specs

The NEXTflex Obesity-2 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina^® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.