NEXTflex® Periodic Fever-2 Amplicon Panel

Validated panel for detection of PSTPIP1, LPIN2, and ELANE genes with library prep reagents and 384 barcodes for Illumina sequencing.

Catalog# Product Name Quantity US List Price
NOVA-4253-01
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (8 Barcodes)
8 rxns $610 Buy Now
NOVA-4253-02
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (48 Barcodes)
48 rxns $3,510 Buy Now
NOVA-4253-03
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (Barcodes 1- 96)
96 rxns $6,724 Buy Now
NOVA-4253-04
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (Barcodes 97 - 192)
96 rxns $6,724 Buy Now
NOVA-4253-05
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (Barcodes 193 -288)
96 rxns $6,724 Buy Now
NOVA-4253-06
NEXTflex® Periodic Fever-2 Amplicon Panel for Illumina® platforms (Barcodes 289 - 384) 96 rxns
$6,724 Buy Now

NEXTflex Periodic Fever-2 Kit Box
  • 100% coverage of PSTPIP1, LPIN2, and ELANE exons and flanking intron-exon boundaries 
  • 98% uniformity of amplicons on 0.2x coverage; 100% of amplicons detected at >0.1X mean coverage
  • 384 unique barcodes available
  • Complete solution for targeted sequencing, including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 20 ng DNA isolated from fresh or frozen samples required

For research use only. Not for use in diagnostic procedures.

Coverage of PSTPIP1, LPIN2, and ELANE Genes for Illumina® Sequencing

The NEXTflex® Periodic Fever-2 Amplicon Panel allows you to quickly target and sequence 43 exons of the PSTPIP1, LPIN2, and ELANE loci. This kit contains primer pairs and reagents needed to amplify these coding regions of PSTPIP1, LPIN2, and ELANE from DNA isolated from fresh or frozen material and prepare libraries for Illumina® sequencing.

While up to 384 barcodes are currently supplied with the NEXTflex Periodic Fever-2 Amplicon Panel Amplicon Panel, the panel has the capability of multiplexing up to 600 samples at 100x coverage for detection of germline mutations on a single Illumina® 2x300 MiSeq® lane. If you need the ability to multiplex more than 384 samples, contact nextgen@biooscientific.com to request a quote for a panel with additional barcodes.

Periodic Fever-2 Table


A Complete Solution for Targeted PSTPIP1, LPIN2, and ELANE Sequencing

The NEXTflex Periodic Fever-2 Amplicon Panel is a complete, validated solution that targets 43 exons in PSTPIP1, LPIN2, and ELANE loci using 62 PCR primer pairs. This kit requires only 20 ng of DNA isolated from fresh or frozen samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® Periodic Fever-2 Amplicon Panel contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from fresh or frozen samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® Periodic Fever-2 Amplicon Panel

Periodic Fever-2 Flow Chart

NEXTflex Periodic Fever-2 Amplicon Panel Kit Contents

NEXTflex® Periodic Fever-2 Amplicon Primer Mix 1
NEXTflex® Periodic Fever-2 Amplicon Primer Mix 2
NEXTflex® PCR Enhancer 1
NEXTflex® PCR Enhancer 2
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

20 - 100 ng of extracted genomic DNA (two 10 - 50 ng aliquots in up to 25 μL nuclease-free water each) 
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research®, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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