NEXTflex™ Rapid Pre-Capture Combo DNA Library Prep Kit for SeqCap EZ Target Capture

Low input library preparation for NimbleGen SeqCap EZ library construction and Illumina sequencing

Catalog# Product Name Quantity US List Price
5144-41 NEXTflex™ Rapid Pre-Capture Combo Kit (24 barcodes) 48 rxns $2,170 Buy Now
5144-42 NEXTflex™ Rapid Pre-Capture Combo Kit (48 barcodes) 96 rxns $4,229 Buy Now
5144-43 NEXTflex™ Rapid Pre-Capture Combo Kit (96 barcodes) 192 rxns $8,235 Buy Now

NEXTflex Rapid Pre-Capture Combo Kit Nimblegen SeqCap EZ

  • Complete kit – Contains library prep, barcodes and barcode blockers compatible with SeqCap EZ target capture
  • Low input - only 10 ng of input DNA required
  • Optimized - Offers larger number of on-target reads
  • Multiplex up to 96 samples with single-index barcodes
  • Reduces the cost of target capture
  • Compatible with Illumina sequencing platforms

 

The NEXTflex™ Rapid Pre-Capture Combo Kit is a complete low-input library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of SeqCap EZ Library target enrichment. This kit facilitates deep multiplexing for both exome target capture and DNA target capture.

 

Component Table for Integrated SeqCap Target Capture

 

The NEXTflex Rapid PreCapture Combo Kit is designed to prepare single read or paired-end libraries for DNA and exome target capture and Illumina sequencing. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads.


Automation for Library Prep for Target Capture

The NEXTflex Rapid Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone NGS, Sciclone NGSx and Sciclone G3  platforms. For more information contact NextGen@biooscientfic.com.


Multiplex up to 96 Samples with SeqCap Target Capture

The NEXTflex™ DNA Barcodes and NEXTflex™ DNA Barcode Blockers for SeqCap are included in the NEXTflex Rapid Pre-Capture Combo Kit and are also available separately. 

 

NEXTflex Rapid Pre-Capture Combo Kit Infographic

 

Our whitepaper written in conjunction with Roche, An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture,  demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep in preparation for Illumina sequencing.


Achieving High Coverage and Yield from GC and AT Rich Genomes

 

The PCR amplification step in library preparation introduces biases including uneven coverage of regions with extreme base composition, increased numbers of duplicate fragments, decreased mapping quality and poor variant calling. In our new white paper, Achieving High Coverage and Yield from GC and AT Rich Genomes, we show that the NEXTflex™ PCR polymerase contains a highly optimized and robust enzyme that exhibits minimal GC bias and produces uniform coverage of difficult to sequence genomes.

 


Publications that Cite Using the NEXTflex Rapid Pre-Capture Combo Kit

Suren, H., et al.  (2016) Exome capture from the spruce and pine giga-genomes. Molecular Ecology Resources Mol Ecol Resour. doi:10.1111/1755-0998.12570.


Kit Specs

The NEXTflex Rapid Pre-Capture Combo Kit contains DNA library preparation reagents to prepare 48, 96 or 192 genomic DNA or exome libraries for SeqCap EZ target capture and downstream Illumina sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.

NEXTflex Rapid Pre-Capture Combo Kit  for SeqCap Target Capture

Rapid Pre-Capture Combo Kit Flow Chart

 

 

 

Kit Contents

NEXTflex™ End-Repair & Adenylation Buffer Mix
NEXTflex™ End-Repair & Adenylation Enzyme Mix 
NEXTflex™ Ligase Enzyme Mix
NEXTflex™ DNA Barcode Adapter 1 - 24 (25 µM)
NEXTflex™ PCR Master Mix
NEXTflex™ Primer Mix (12.5 µM)
NEXTflex™ INV-HE Index 1 – 24 (250 µM)
NEXTflex™ HE Universal Oligo 1 (500 µM)
NEXTflex™ LM-PCR Oligo 1 (100 µM)
NEXTflex™ LM-PCR Oligo 2 (100 µM)
NEXTflex™ LM-PCR Master Mix 
Nuclease-free Water
Resuspension Buffer

Required Materials not Provided

10 ng - 1 µg fragmented genomic DNA in up to 32 µL nuclease-free water.
NimbleGen SeqCap EZ Library and related consumables
NimbleGen SeqCap EZ Hybridization and Wash Kit (Roche, Cat. # 05634261001, 24
reactions) and related consumables
NimbleGen SeqCap Pure Capture Bead Kit (Roche, Cat. # 06977952001, 24 reactions) and
related consumables
For Human Studies: COT Human DNA (Roche, Cat. # 11581074001)
For Non-Human Studies: Nimblegen SeqCap EZ Developer Reagent (Roche, Cat. #
06684335001)
Ethanol 100% (room temperature)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Agencourt AMPure XP 60 mL (Beckman Coulter Genomics, Cat # A63881)
Magnetic Stand -96 (Ambion, Cat # AM10027) or similar
Heat block
Thermocycler capable of holding 47ºC for 72 hours
2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge
1.5 mL nuclease-free microcentrifuge tubes
Vortex

Sequences of NEXTflex DNA Barcode Indexes - Excel / PDF

Sequences of NEXTflex-96 DNA Barcode Indexes - Excel / PDF

Instructions for installing NEXTflex Barcode Indices in Illumina Experiment Manager

 

Download the whitepaper, An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture