NEXTflex® TP53 Amplicon Panel for FFPE

Validated TP53 panel with library prep reagents and 384 barcodes for Illumina® sequencing

Catalog# Product Name Quantity US List Price
NOVA-4252-01
NEXTflex® TP53 Amplicon Panel for FFPE (8 Barcodes)
8 rxns $313 Buy Now
NOVA-4252-02
NEXTflex® TP53 Amplicon Panel for FFPE (48 Barcodes)
48 rxns $1,730 Buy Now
NOVA-4252-03
NEXTflex® TP53 Amplicon Panel for FFPE (Barcodes 1- 96)
96 rxns $3,164 Buy Now
NOVA-4252-04
NEXTflex® TP53 Amplicon Panel for FFPE (Barcodes 97 - 192)
96 rxns $3,164 Buy Now
NOVA-4252-05
NEXTflex® TP53 Amplicon Panel for FFPE (Barcodes 193 -288)
96 rxns $3,164 Buy Now
NOVA-4252-06
NEXTflex® TP53 Amplicon Panel for FFPE (Barcodes 289 - 384) 96 rxns
$3,164 Buy Now

NEXTflex® TP53 for FFPE Kit Box
  • Complete TP53 coverage
  • 99% uniformity of all targeted coding exons and exon-intron boundaries, validated on DNA isolated from FFPE samples
  • Complete solution for targeted sequencing including gene specific primers, PCR Master Mix, clean-up beads, and up to 384 barcodes
  • Low input - Only 40 ng DNA isolated from FFPE samples required

For Research Use Only. Not for use in diagnostic procedures.

Complete TP53 Coverage for Illumina® Sequencing of FFPE DNA

The NEXTflex® TP53 Amplicon Panel for FFPE allows you to quickly target and sequence all coding exons of the TP53 locus. This kit contains 27 primer pairs and reagents needed to amplify the coding regions of TP53 from DNA isolated from FFPE material and prepare libraries for Illumina® sequencing.

The NEXTflex TP53 Amplicon Panel for FFPE has the capability of multiplexing up to 50 samples at 2,000x coverage for detection of somatic mutations on a single Illumina® 2x300 MiSeq® lane.

TP53 for FFPE Table


A Complete Solution for Targeted TP53 Sequencing

The NEXTflex TP53 Amplicon Panel for FFPE is a complete solution, validated with DNA isolated from FFPE samples, that targets all coding exons in the TP53 locus using 27 PCR primer pairs. This kit requires only 40 ng of DNA isolated from FFPE samples. The convenient predesigned panel allows researchers to focus on data generation and analysis, not on labor-intensive primer design and target selection. This kit can be easily automated on liquid handlers, further simplifying workflow.


Kit Specs

The NEXTflex® TP53 Amplicon Panel for FFPE contains enough material to prepare 8, 48, or 96 samples for Illumina® sequencing from DNA isolated from FFPE-fixed samples. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.

NEXTflex® TP53 Amplicon Panel for FFPE

TP53 FFPE Flow Chart

NEXTflex TP53 Amplicon Panel for FFPE Kit Contents

NEXTflex® TP53 Amplicon FFPE Primer Mix 1-4
NEXTflex® PCR Enhancer 1
NEXTflex® Hot Start PCR I Master Mix (A)
NEXTflex® Ligation Mix
NEXTflex® Amplicon DNA Adapter
NEXTflex® PCR II Barcoded Primer Mix
NEXTflex® PCR II Master Mix
Nuclease-free Water
Resuspension Buffer
NEXTflex® Cleanup Beads

Required Materials Not Provided

10 - 50 ng of DNA isolated from FFPE samples (four 10 – 50 ng aliquots in up to 29 µL nuclease-free water each)
Ethanol 80% (room temperature) 
96 well PCR Plate Non-skirted (Phenix Research®, Cat # MPS-499) or similar 
96 well Library Storage and Pooling Plate (Fisher Scientific®, Cat # AB-0765) or similar 
Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001) 
Magnetic Stand -96 (Thermo Fisher Scientific®, Cat # AM10027) or similar 
Thermocycler 
2, 10, 20, 200 and 1000 μL pipettes / multichannel pipettes 
Nuclease-free barrier pipette tips 
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes 
Vortex

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For Research Use Only. Not for use in diagnostic procedures.