NextPrep-Mag™ cfDNA Isolation Kit 

• Ideal for extracting DNA from human plasma for cell-free DNA-Seq
• Generates highly concentrated libraries using few PCR cycles
• Automation friendly, mag-bead based format
• Fast, 30 minute protocol with minimal hands-on time
• Ideal for NGS library prep
• No vacuum pumps, vacuum manifolds, or column extenders required

 

The NextPrep-Mag™ cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from human blood plasma or serum, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex Cell Free DNA-Seq Library Prep Kit for Illumina^® Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Torrent Sequencing. This kit is optimized for isolating circulating tumor and cell free circulating fetal DNA from blood plasma.

The NextPrep-Mag™ cfDNA automated isolation kit is an all comprehensive high-throughput solution for cfDNA extraction from plasma and serum.

For research use only. Not for use in diagnostic procedures.

Cell Free DNA Isolation Protocol

This cell free DNA isolation procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.

Cell Free DNA Isolation Challenges

Extraction of DNA from plasma is challenging because the DNA is highly fragmented and present at very low concentration, especially in healthy individuals. Because the expected yields are low, the DNA recovered cannot be reliably detected using standard methods such as NanoDrop spectrophotometer, gel or capillary electrophoresis, or DNA binding dyes.

Kit Specs

The NextPrep-Mag™ cfDNA Isolation Kit For < 1 mL - 3 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of 1 mL plasma or less, or 16 samples of 3 mL plasma. The NextPrep-Mag™ cfDNA Isolation Kit For 3 mL - 5 mL Plasma Samples contains sufficient reagents for extraction of DNA from 50 samples of plasma ranging from 3 mL to 5mL. The kit components are stable for at least 12 months from time of receipt, when properly stored.