The NEXTflex™ Pre-Capture Combo Kit (NimbleGen SeqCap EZ Library Compatible) is a complete Illumina-compatible library prep solution designed for DNA library preparation, multiplexing, and barcode blocking, upstream of Roche NimbleGen’s SeqCap EZ Library target enrichment. This kit facilitates deep multiplexing for both exome target capture and DNA target capture.
The NEXTflex PreCapture Combo Kit (NimbleGen SeqCap EZ Library Compatible) is designed to prepare single, paired-end, and multiplexed genomic DNA libraries for DNA and exome target capture and Illumina compatible sequencing. This kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. With these improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and higher binding efficiencies. The NEXTflex Pre-Capture Combo Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation.
The NEXTflex Pre-Capture Combo Kit is compatible with Beckman Biomek® FX and Biomek® FXP Laboratory Automation Workstations. A program is currently being validated for use with the Sciclone NGS, Sciclone NGSx and Sciclone G3 platforms. For more information contact NextGen@biooscientfic.com.
Increased Multiplexing Capabilities
The NEXTflex™ DNA Barcodes and NEXTflex™ DNA Barcode Blockers for SeqCap are included in the NEXTflex PreCapture Combo Kit and are also available separately.
Our whitepaper written in conjunction
with Roche NimbleGen, An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture, demonstrates the efficacy of this kit when used with the NimbleGen SeqCap EZ Library system for library prep in preparation for Illumina sequencing.
Selected Publications that Reference Using the NEXTflex Pre-Capture Combo Library Prep Kit:
Kocak, et al (2014) Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1. Genes and Development. 28:2090-2102.
Koçak, H. (2014) Functional Analysis of ACD Mutations in Dyskeratosis Congenita (Unpublished doctoral dissertation). The University of Michigan.
McCarthy, SE, et. al. (2014) De novo mutations in schizophrenia implicate chromatin remodeling and support a genetic overlap with autism and intellectual disability. Mol. Psychiatry 19:652-8.
Yeh, I. et al. (2015) Activating MET kinase rearrangements in melanoma and Spitz tumours. Nature Communications. 6:7174, doi:10.1038/ncomms8174.
The NEXTflex PreCapture Combo Kit (NimbleGen SeqCap EZ Library Compatible) contains DNA-seq library preparation reagents to prepare 48, 96, 192, 384 or 768 genomic DNA-seq or exome libraries for Roche NimbleGen’s SeqCap EZ Library capture systems and downstream Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.