Bioo Scientific’s NEXTflex™ Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina® sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientific’s NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, Optimizing Library Preparation: Enhanced Adapter Ligation Technology, demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina® sequencing.
Cost Effective NEXTflex Rapid DNA-Seq Kit Bundles
To simplify your DNA sequencing Bioo Scientific now offers affordable bundles containing NEXTflex Rapid DNA-Seq library prep reagents and NEXTflex DNA Barcodes. There are two different versions of this kit. They each contain the 48 rxn NEXTflex Rapid DNA-Seq Kit and 24 unique NEXTflex DNA Barcodes (in aliquots of 2 reactions each).
Flexible Multiplexing Options
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex™ DNA Barcodes, NEXTflex-96™ DNA Barcodes and NEXTflex-HT™ Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex™ ChIP-Seq Barcodes and NEXTflex-96™ ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Library Prep Kit was designed for easy migration onto automated liquid handling platforms. Currently methods are available for the Beckman-Coulter Biomek® FX and Biomek® FXP Laboratory Automation Workstations. Additionally a validated automation protocol for use with the PerkinElmer Sciclone G3 NGS platform is now available. Download the Sciclone NGS and NGSx Workstation Automation Guides for the NEXTflex Rapid DNA-Seq Kit.
Publications that Cite Using the NEXTflex Rapid DNA-Seq Kit
Brodie, J., et al. (2016) Characterising the microbiome of Corallina officinalis, a dominant calcified intertidal red alga. FEMS Microbiology Ecology. doi: 10.1093/femsec/fiw110.
Campana, M. G., et al. (2014) False positives complicate ancient pathogen identifications using high-throughput shotgun sequencing. BMC Research Notes. 7:111 http://www.biomedcentral.com/1756-0500/7/111.
Campbella, M. A., et al. (2015) Genome expansion via lineage splitting and genome reduction in the cicada endosymbiont Hodgkinia. PNAS. doi: 10.1073/pnas.1421386112.
Cervera-Juanes, R., et al. (2015) MAOA expression predicts vulnerability for alcohol use. Molecular Psychiatry. doi:10.1038/mp.2015.93.
Chwialkowska, K., Nowakowska, U., Mroziewicz, A., Szarejko, I., and Kwasniewski, M. (2016) Water-deficiency conditions differently modulate the methylome of roots and leaves in barley (Hordeum vulgare L.) J. Exp. Bio. doi: 10.1093/jxb/erv552.
Devall, M., et al. (2015) A comparison of mitochondrial DNA isolation methods in frozen post-mortem human brain tissue—applications for studies of mitochondrial genetics in brain disorders. Biotechniques 59(4): 241-246.
DeWitt, M., Magis, W., Bray, N. et al. (2016, Jan 15). Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex Retrieved from http://biorxiv.org/content/early/2016/01/15/036236
Gal, C., et al. (2015) Abo1, a conserved bromodomain AAA‐ATPase, maintains global nucleosome occupancy and organization. EMBO Reports. 16:11. pp 1409 – 1580. doi: 10.15252/embr.201540476.
Gal, C. et. al. (2015) The impact of the HIRA histone chaperone upon global nucleosome architecture. Cell Cycle. 14:1, 123-134. doi:10.4161/15384101.2014.967123.
Gan, H. M., Tan, M. H., Lee, Y. P. and Austin, C. M. (2014) The complete mitogenome of the Australian tadpole shrimp Triops australiensis (Spencer & Hall, 1895) (Crustacea: Branchiopoda: Notostraca).
Gan, H. M., Tan, M. H., Lee, Y. P. and Austin, C. M. (2014) The complete mitogenome of the river blackfish, Gadopsis marmoratus (Richardson, 1848) (Teleostei: Percichthyidae). Mitochondrial DNA. doi:10.3109/19401736.2014.974174.
Givnish, T. J., et al. (2016) Phylogenomics and historical biogeography of the monocot order Liliales: out of Australia and through Antarctica. Cladistics. doi: 10.1111/cla.12153.
Goh, H. F. and Philip, K. (2015) Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin. PLoS ONE. doi: 10.1371/journal.pone.0140434.
Gultekin, S. E., et al. (2016) Unusual Presentation of an Adenocarcinoma of the Lung Metastasizing to the Mandible, Including Molecular Analysis and a Review of the Literature. Journal of Oral and Maxillofacial Surgery, doi:10.1016/j.joms.2016.06.004.
Harrisson, K., et al. (2016) Pleistocene divergence across a mountain range and the inﬂuence of selection on mitogenome evolution in threatened Australian freshwater cod species. Heredity. 1–10. doi:10.1038/hdy.2016.8.
Hill, C. J. et al. (2016) Effect of room temperature transport vials on DNA quality and phylogenetic composition of faecal microbiota of elderly adults and infants. Microbiome. 4:19. doi: 10.1186/s40168-016-0164-3.
Laver, T., Harrison, J., O’Neill, P. A., Moore, K., Farbos, A., Paszkiewicz, K. and Studholme, D. J. (2015) Assessing the performance of the Oxford Nanopore Technologies MinION. Biomolecular Detection and Quantification 3 (2015) 1–8. doi :10.1016/j.bdq.2015.02.001.
Palomo, A., et al. (2016) Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp. The ISME Journal. doi:10.1038/ismej.2016.63.
Shain, A. H., et al. (2015) The Genetic Evolution of Melanoma from Precursor Lesions. The New England Journal of Medicine. 373:1926-1936. doi: 10.1056/NEJMoa1502583.
Tatarenkova, A., Mesaka F. and Avisea, J. C. (2015) Complete mitochondrial genome of a self-fertilizing fish Kryptolebias marmoratus (Cyprinodontiformes, Rivulidae) from Florida. Mitochondrial DNA. doi: 10.3109/19401736.2015.1115861.
Yang, Y. A., et al. (2016) FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function. Nucleic Acids Research. doi: 10.1093/nar/gkw498.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina® sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C. These kits ship on dry ice.