NEXTflex™ DNA Library Prep Modules

For Illumina DNA library prep

Catalog# Product Name Quantity US List Price
5140-05 NEXTflex™ DNA End Repair Mix 100 rxns $322 Buy Now
5140-06 NEXTflex™ DNA Adenylation Mix 100 rxns $432 Buy Now
5140-07 NEXTflex™ DNA Ligation Mix 100 rxns $1,087 Buy Now
5140-08 NEXTflex™ DNA PCR Master Mix 100 rxns $677 Buy Now


  • Flexible options – NEXTflex™ DNA Modules are available in aliquots containing any number of reactions you need
  • Convenient – Reactions are provided in master mix format to reduce steps during DNA sample prep workflows
  • Enhanced Adapter Ligation Technology offers larger number of unique sequencing reads
  • Automation-friendly workflow is compatible with liquid handlers
  • Compatible with targeted sequence capture
  • Functionally validated with Illumina sequencing platforms

 

The NEXTflex™ DNA Modules offer additional flexibility to Illumina library preparation. All of these modules are compatible with Illumina sequencing platforms. These modules are available in any quantity greater than 100 reactions. 


NEXTflex™ DNA End Repair Module 

The NEXTflex™ DNA End-Repair Module is designed with enzymes and buffers to efficiently repair fragmented DNA with overhangs to blunt ended DNA. The NEXTflex™ DNA End Repair Module is supplied as a master mix to maximize efficiency and convenience in DNA sample preparation workflows. 


NEXTflex™ DNA Adenylation Module

The NEXTflex™ DNA Adenylation Module is designed with enzymes and buffers to efficiently incorporate an “A” on the 3’ end of blunt DNA fragments.


NEXTflex™ DNA Ligation Module

The NEXTflex™ DNA Ligation Module is designed with enzymes and buffers to efficiently ligate DNA adapters to adenylated DNA fragments. Using Bioo Scientific’s Enhanced Ligation Technology, adapters are bound to more DNA fragments leading to increased sequencing diversity and reads.


NEXTflex™ DNA PCR Module

The NEXTflex™ DNA PCR Module is designed with enzymes, buffers and nucleotides for efficient amplification of adapter ligated DNA.


Selected Publications that Cite Using the NEXTflex DNA Modules

Kloth, M., et al. (2015) Activating ERBB2/HER2 mutations indicate susceptibility to pan-HER inhibitors in Lynch and Lynch-like colorectal cancer. Gut doi:10.1136/gutjnl-2014-309026.

König, K. et al. (2015) Implementation of Amplicon Parallel Sequencing Leads to Improvement of Diagnosis and Therapy of Lung Cancer Patients. Journal of Thoracic Oncology. 10 :7, 1049–1057. doi: 10.1097/JTO.0000000000000570.

Lundberg, p. et al. (2014) Clonal evolution and clinical correlates of somatic mutations in myeloproliferative neoplasms. Blood, 123:4 pp. 2220-8.

Merlevede, J. et al. (2016) Mutation allele burden remains unchanged in chronic myelomonocytic leukaemia responding to hypomethylating agents. Nat. Commun. 7:10767 doi: 10.1038/ncomms10767.


For larger volume requirements, customization and bulk packaging is available. Please contact nextgen@biooscientific.com for further information. 

Modules are also available for PCR-Free DNA-seq, ChIP-seq, RNA-seq, and directional RNA-seq library preparation


Kit Specs

The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.