- 96 barcode blockers available
- Index-specific blocking oligos result in a higher % of on-target reads
- Compatible with NEXTflex™ DNA Barcodes and NEXTflex™-96 DNA Barcodes
- Validated in combination with Roche NimbleGen’s SeqCap EZ library and Agilent's SureSelect XT2 target capture kits for Illumina sequencing
- Simplifies and reduces the cost of target capture
The NEXTflex™ DNA Barcode Blockers are designed for use with the NEXTflex™ DNA Barcodes and NEXTflex-96™ DNA Barcodes. Both the barcodes and blockers are compatible with Roche NimbleGen’s SeqCap EZ library and Agilent's SureSelectXT2 target capture systems. Other solution-based hybridization systems are also compatible, yielding varying numbers of reactions per kit. The NEXTflex DNA Barcode Blockers are available in sets of 6, 12, 24, 48 or 96 blocking oligos, and are designed to prevent concatemerization of non-complimentary library fragments during solution-based hybridization assays. Alternatively, complete library preparation, barcode and blocker solutions are available. The NEXTflex™ Pre-Capture Combo Kit (NimbleGen SeqCap) includes NEXTflex DNA library prep, NEXTflex DNA Barcodes for multiplexing and the NEXTflex Barcode Blockers. The NEXTflex™ Pre- and Post- Capture Combo Kit (Agilent SureSelectXT2 Compatible) includes NEXTflex Rapid DNA library prep reagents, NEXTflex DNA Barcodes and the NEXTflex Barcode Blockers.
The NEXTflex-96 Barcode Blockers are the most affordable blocking solution for SeqCap target capture
The NEXTflex-96 Barcode Blockers allow for multiplexing up to 96 samples in 192 target capture reactions, for a total of 18,432 individual libraries per kit (96 libraries x 192 target capture reactions = 18,432 samples processed). This translates to a cost of only $0.58 per individual library.
Whitepapers are available demonstrating the efficacy of the NEXTflex DNA Barcode Blockers for improved target capture specificity, for the construction of high quality libraries using the NimbleGen SeqCap EZ Library and Agilent's SureSelectXT2 target capture systems.
The NEXTflex™ DNA Barcode Blockers contain 6, 12, 24, 48 or 96 oligo blockers designed to be used in conjunction with NEXTflex™ DNA Barcodes. These kits ship on dry ice.
NEXTflex™ HE Universal Oligo 1
NEXTflex™ INV-HE Index Oligos