Custom AIR™ Adenylated Linkers

For small RNA-seq library prep



  • Ideal for next-generation sequencing, miRNA cloning and miRNA sequencing
  • 3’ end blocks prevent self-ligation
  • Adenylated 5' end reacts with T4 RNL2 Ligase, truncated
  • Phosphorothioate and other modifications available
  • Predesigned linkers are also available
  • You can create your own miRNA barcodes 

 

To request a quote for custom adenylated adapters call 512-707-8993 or email us at nextgen@biooscientific.com.

Design your own miRNA cloning linkers.

Bioo Scientific’s AIR™ Adenylated Linkers are 5’adenylated oligos with 3’end blocks, which are ready for use in NGS or miRNA cloning. Custom adaptors can be designed for use with any NGS platform.

AIR™ Adenylated Linkers are activated adenylated oligos that preclude the need for ATP in RNA Ligase 2 (RNL2) reactions. This allows for specific and clean ligations.

 

Selected Publications that Cite the Use of Custom AIR Adenylated Linkers:

Alekseyenko, A. A., et al. (2015) UNIT 21.30 BioTAP-XL: Cross-linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin-Associated Complexes. Current Protocols in Molecular Biology. doi: 10.1002/0471142727.mb2130s109.

Benes, V., et al. (2015) Identification of cytokine-induced modulation of microRNA expression and secretion as measured by a novel microRNA specific qPCR assay. Scientific Reports. 5:11590. doi:10.1038/srep11590.

Haenni, S. et al. (March 2012) Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq. Nuc Acid Res, 40:6 http://nar.oxfordjournals.org/content/early/2012/03/28/nar.gks282.full

 

Kit Specs

Available in 1 nm to 1 µm quantities

Most custom AIR Adenylated Linkers have a 10 day turnaround time.

Validated on an Illumina® sequencing platform