The NEXTflex® Rapid Directional RNA-Seq Kit provides an easy and flexible solution for generating single read, paired-end and multiplexed stranded libraries from mRNA or rRNA depleted total RNA. This kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from with >99% strand specificity. This stranded information improves transcript annotation and improves read alignment, reducing per sample sequencing costs. dUTP-based strand orientation also enables the detection of antisense expression regulatory relationships and allows for the discovery of novel ncRNA.
Cost Effective, Stranded RNA Library Prep Kit Bundles
To simplify your mRNA sequencing, Bioo Scientific now offers affordable bundles containing the NEXTflex Rapid Directional RNA-Seq library prep reagents, NEXTflex RNA-Seq Barcodes and NEXTflex Poly(A) Beads. There are two different versions of this kit. They each contain the 48 rxn NEXTflex Rapid Directional RNA-Seq Kit, NEXTflex Poly(A) Beads (48 rxns) and 24 unique NEXTflex RNA-Seq Barcodes (in aliquots of 2 reactions each).
Improved Rapid Stranded RNA-Seq Offers Higher Library Yields and Better Mapped Reads
The whitepaper, Improved Rapid Stranded RNA-Seq Offers Higher Library Yields and Better Mapped Reads, illustrates a detailed comparison of the NEXTflex Rapid Directional RNA-Seq Kit with a competitor’s fast directional library prep kit. The NEXTflex Rapid Directional RNA-Seq Kit excelled at rRNA removal, library yield and overall quality metrics.
Robust Library Prep with Low Input Amounts of Total RNA
Bioo Scientific’s whitepaper, Constructing High Quality RNA-Seq Libraries from Limited Amounts of Total RNA, illustrates the robustness of the NEXTflex™ Rapid Directional RNA-Seq Kit used along with NEXTflex Poly(A) Beads for mRNA isolation and library preparation from input as low as 10 ng of total RNA.
Automation Protocols for Stranded RNA Library Prep
Optimized automation protocols for stranded RNA library prep using the NEXTflex Rapid Directional RNA-Seq Library Preparation Kits are available for the Sciclone® NGS and NGSx Workstations. Download the Sciclone NGS and NGSx Workstation Automation Guides for the NEXTflex Rapid Directional RNA-Seq Kit.
Flexible Multiplexing Options for Illumina® RNA Sequencing
The NEXTflex Rapid Directional RNA-Seq Library Prep Kit was designed to be used in conjunction with the NEXTflex™ RNA-Seq Barcodes or NEXTflex-96™ RNA-Seq Barcodes for multiplexing. These single index barcodes are available in sets of 6, 12, 24, 48 and 96 barcodes.
Poly(A) Beads for mRNA Purification
The NEXTflex™ Poly(A) Beads offer a convenient method, validated with the NEXTflex Rapid Directional RNA-Seq Kit, for purification of pure, intact mRNA.
Selected Publications that Reference Using the NEXTflex Rapid Directional RNA Library Prep Kit:
Beyaz, S. et al. (2016) High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 531, 53–58. doi:10.1038/nature17173.
Blance, S., et al. (2016) Stem cell function and stress response are controlled by protein synthesis. Stem cell function and stress response are controlled by protein synthesis. Nature, 534, 335–340. doi:10.1038/nature18282.
Bonizzoni, M., et al. (2013) Probing functional polymorphisms in the dengue vector, Aedes aegypti. BMC Genomics. 14:739 http://www.biomedcentral.com/1471-2164/14/739.
Carnes, M. U., et al. (2015) The Genomic Basis of Postponed Senescence in Drosophila melanogaster. PLoS ONE. doi: 10.1371/journal.pone.0138569.
Denzler, R., Agarwal V., Stefano J., Bartel DP. and Stoffel, M. (2014) Assessing the ceRNA hypothesis with quantitative measurements of miRNA and target abundance. Mol. Cell. 54:4 pp 766-76.
Dharshinia, S., et al. (2016) De novo sequencing and transcriptome analysis of a low temperature tolerant Saccharum spontaneum clone IND 00-1037. J of Biotechnology. doi: 10.1016/j.jbiotec.2016.05.036.
Dobáková, E., Flegontov, P., Skalický, T. and Lukeš, J. (2015) Unexpectedly Streamlined Mitochondrial Genome of the Euglenozoan Euglena gracilis. Genome Biol Evol. 7. 3358-3367. doi:10.1093/gbe/evv229.
Fang, W. and Bartel, D. P. (2015) The Menu of Features that Define Primary MicroRNAs and Enable De Novo Design of MicroRNA Genes. Molecular Cell. 60:1. p131–145. doi: 10.1016/j.molcel.2015.08.015.
Guérin, F., Isnard, C., Cattoir, V. and Giard, J. C. (2015) Complex Regulation Pathways of AmpC-Mediated β-Lactam Resistance in Enterobacter cloacae Complex. Antimicrob. Agents Chemother., 59: 7753 - 7761. doi: 10.1128/AAC.01729-15.
Jones, B. M., Wcislo, W. T. and Robinson, G. E. (2015) Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis. g3, Oct 2015; 5: 2127 - 2135. doi: g3.115.021261v1.
Lamanna, F., et al. (2015) Cross-tissue and cross-species analysis of gene expression in skeletal muscle and electric organ of African weakly-electric fish (Teleostei; Mormyridae). BMC Genomics. 16:668 . doi:10.1186/s12864-015-1858-9.
Lin, M.-H., Jones, D. F. and Fleming, R. (2015) Transcriptomic analysis of degraded forensic body fluids, Forensic Science International: Genetics, Volume 17. 35-42. dio: 10.1016/j.fsigen.2015.03.005.
McNeill, M. S., Kapheim, K. M., Brockmann, A., McGill, T. A. W., Robinson, G. E. (2015) Brain regions and molecular pathways responding to food reward type and value in honey bees. Genes, Brain and Behavior. doi: 10.1111/gbb.12275.
Mullenders, J., et al. (2015) Cohesin loss alters adult hematopoietic stem cell homeostasis, leading to myeloproliferative neoplasms. J. Exp. Med. 2015; 212:1833-1850. doi: 10.1084/jem.20151323.
Nam, J., Rissland, O.S., Koppstein, D. et al. (2014) Global Analyses of the Effect of Different Cellular Contexts on MicroRNA Targeting. Molecular Cell. http://dx.doi.org/10.1016/j.molcel.2014.02.013.
Pham, K. T. M., et al. (2015) MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis. PLOS Genetics. doi: 10.1371/journal.pgen.1005385.
Rittschofa, C. C., et al. (2014) Neuromolecular responses to social challenge: Common mechanisms across mouse, stickleback fish, and honey bee. PNAS. doi: 10.1073/pnas.1420369111.
Rossetto CC, Tarrant-Elorza M, Pari GS (2013) Cis and Trans Acting Factors Involved in Human Cytomegalovirus Experimental and Natural Latent Infection of CD14 (+) Monocytes and CD34 (+) Cells. PLoS Pathog 9(5): e1003366. doi:10.1371/journal.ppat.1003366.
Rube, H. T., et al. (2016) Sequence features accurately predict genome-wide MeCP2 binding in vivo. Nature Communications. 7:11025. doi:10.1038/ncomms11025.
Solovchenko, A., et al. (2016) Nitrogen availability modulates CO2 tolerance in a symbiotic chlorophyte, Algal Research, 16, 177-188. doi: 10.1016/j.algal.2016.03.002.
Tarvin, R. D., Santos, J. C., O'Connell, L. A., Zakon, H. H. and Cannatella, D. C. (2016) Convergent Substitutions in a Sodium Channel Suggest Multiple Origins of Toxin Resistance in Poison Frogs. Mol Biol Evol. 33:4. 1068-1081. doi: 10.1093/molbev/msv350.
Van Laar, T, A., Chen, T, You, T., and Leung, K. P. (2015) Sublethal Concentrations of Carbapenems Alter Cell Morphology and Genomic Expression of Klebsiella pneumoniae Biofilms. Antimicrobial Agents and Chemotherapy. doi: 10.1128/AAC.04581-14.
Wheeler, M. M. and Robinson G. E. (2014) Diet-dependent gene expression in honey bees: honey vs. sucrose or high fructose corn syrup. Scientific Reports. 4: 5726. doi:10.1038/srep05726.
Zhang, Y. et al. (2015) Genome-, Transcriptome- and Proteome-Wide Analyses of the Gliadin Gene Families in Triticum urartu. PLOS One. doi: 10.1371/journal.pone.0131559.
The NEXTflex Rapid Directional RNA Library Prep Kit contains enough material to prepare 8 or 48 RNA samples for Illumina® sequencing. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at -20°C.