NEXTflex™ Ion Torrent Compatible DNA-Seq Library Prep Kits 

DNA-Seq LIbrary Prep for Ion S5, Ion S5 XL, Ion Torrent PGM and Ion Proton

Catalog# Product Name Quantity US List Price
4001-01 NEXTflex™ DNA-Seq Kit - Ion Torrent Compatible
8 rxns $176 Buy Now
4001-02 NEXTflex™ DNA-Seq Kit - Ion Torrent Compatible 48 rxns $835 Buy Now
401001 NEXTflex™ DNA Barcodes - Ion Torrent Compatible - 8
48 rxns $390 Buy Now
401002 NEXTflex™ DNA Barcodes - Ion Torrent Compatible - 16
96 rxns $779 Buy Now
401003 NEXTflex™ DNA Barcodes - Ion Torrent Compatible - 32
192 rxns $1,558 Buy Now
401004 NEXTflex™ DNA Barcodes - Ion Torrent Compatible - 64
384 rxns $3,116 Buy Now

NEXtflex Ion PGM DNA-Seq library prep kit
  • Enhanced Adapter Ligation Technology offers larger number of unique sequencing reads
  • Flexible barcode options– Kits contain 8, 16, 32, or 64 unique barcodes
  • Simplified workflow with 96-well plate compatible volumes
  • Ideal for Ion Torrent based ChIP-seq and DNA-seq library prep

The NEXTflex™ DNA Sequencing Kit - Ion Torrent Compatible is designed to prepare genomic DNA-Seq or ChIP-Seq NGS libraries for sequencing using the Ion S5, Ion S5 XL, Ion Torrent PGM, and Ion Proton sequencing platforms. The NEXTflex DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. This DNA-Seq library prep kit simplifies the Ion Torrent-based library prep workflow by using master mixed reagents and gel-based cleanup. In addition, the option of performing the reactions in a pre-prepared 96-well plate makes this kit more automation friendly than the other available versions. 


Ion S5, Ion PGM and Ion Proton DNA-Seq Multiplexing Options

The NEXTflex™ DNA Barcodes - Ion Torrent Compatible are adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. This format enables multiplexing of up to 64 samples. The NEXTflex DNA Barcodes are available in sets of 8, 16, 32, and 64 unique adapters. The ability to pool samples in an efficient way significantly decreases hands-on time while providing robust data quality.


Selected Publications Citing the Use of the NEXTflex DNA Sequencing Kit for Ion Torrent Sequencing

Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.


Kit Specs

The NEXTflex Ion Torrent DNA-Seq Library Prep Kits contain the reagents to prepare 8 or 48 genomic or ChIP DNA samples for Ion Torrent compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.

Kit Contents

NEXTflex™ End Repair Buffer Mix 
NEXTflex™ End Repair Enzyme Mix 
NEXTflex™ Ligation Mix
NEXTflex™ Adapter Mix (6.25 µM)
NEXTflex™ Primer Mix (12.5 µM)
NEXTflex™ 5X PCR Master Mix
6X Loading Dye
MW Ladder Ready-to-Load 100 bp
Column Elution Buffer
Nuclease-free Water
Resuspension Buffer
DNA Binding Buffer
5X DNA Wash Buffer
Clean-Up Spin Columns


Required Materials not Provided

10 ng, 100 ng or 1 µg of fragmented genomic DNA in up to 40 µL nuclease-free water.
NEXTflex™ DNA Barcodes Ion Torrent Compatible – 8 / 16 / 32 / 64 (Cat # 4010-01, 4010-02, 4010-03, 4010-04)
Ethanol 100% (room temperature)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar 
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
Heat block
Thermocycler 
2, 10, 20, 200 and 1000 mL pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
Microcentrifuge 
1.5 mL nuclease-free microcentrifuge tubes
Low melt agarose such as Low Gelling Temperature Agarose with a melt point of 65ºC (Boston Bioproducts, Cat # P-730) / or / E-gel system
1X TAE buffer
Clean razor or scalpel
SYBR Gold (Invitrogen, Cat # S11494)
UV transilluminator or gel documentation instrument
Gel electrophoresis apparatus
Electrophoresis power supply
Vortex