- Up to 64 samples can be multiplexed
- Compatible with DNA-Seq and ChIP-Seq library prep on the Ion S5, Ion S5 XL, Ion Torrent PGM, and Ion Proton sequencing platforms
- Considerably reduce your per-sample sequencing cost using barcoded multiplexing
- Increase your sequencing scale by pooling 100s of samples on a single flow cell
The Ion Torrent compatible NEXTflex™ DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes use an indexed adapter with a 10 nt unique sequence, allowing for proper differentiation between samples and preventing poor reads caused by single base errors introduced during PCR. Up to 64 samples can be multiplexed using these adapters. The NEXTflex DNA Barcodes Ion Torrent Compatible undergo stringent quality controls and are functionally validated by sequencing.
These barcodes are compatible with the NEXTflex DNA Sequencing Kits Ion Torrent Compatible, the NEXTflex Cell Free DNA-Seq Kit Ion Torrent Compatible, and other genomic and ChIP-Seq DNA library prep protocols compatible with the Ion Torrent PGM, Ion Proton, Ion S5, and Ion S5 XL sequencers.
For larger volume requirements, customized and bulk packaging is available. Please contact firstname.lastname@example.org for further information.
Selected Publications that Reference Using the NEXTflex DNA Barcodes for Ion Torrent Multiplexing:
Appenzeller, S. et al. (2015) Immunoglobulin rearrangement analysis from multiple lesions in the same patient using Next Generation Sequencing. Histopathology. doi: 10.1111/his.12714.
Argueta, W. C. (2015) Comparison of Next Generation Sequencing Methodology on the Ion PGM System Performance versus that on the Sanger Sequencing Method for HV1 and HV2 Regions of mtDNA. University of North Texas Health Science Center. UNTHSC Scholarly Repository.
Lundberg, P. et al. (2014) Clonal evolution and clinical correlates of somatic mutations in myeloproliferative neoplasms. Blood 123(14): 2220-2228.
Raphael, B. H., Shirey, T. B., Lúquez, C. and Maslanka, S. E. (2014) Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains. BMC Microbiol. 192: 14.
The NEXTflex DNA Barcodes Kits Ion Torrent Compatible contain 8, 16, 32, or 64 unique barcodes, enabling the user to multiplex up to 64 samples per flow cell lane. These kits ship on dry ice.
NEXTflex™ DNA P1 Adapter (6.25 µM)
NEXTflex™ DNA Barcode Adapters (6.25 µM)
NEXTflex™ Primer Mix (12.5 µM)
Required Materials not Provided
10 ng, 100 ng or 1 µg of fragmented genomic DNA in up to 40 µL nuclease-free water.
NEXTflex™ DNA-Seq Kit Ion Torrent Compatible or NEXTflex™ Cell Free DNA-Seq Kit Ion Torrent Compatible
Ethanol 100% (room temperature)
Ethanol 80% (room temperature)
96 well PCR Plate Non-skirted (Phenix Research, Cat # MPS-499) / or / similar
96 well Library Storage and Pooling Plate (Fisher Scientific, Cat # AB-0765) / or / similar
Adhesive PCR Plate Seal (BioRad, Cat # MSB1001)
Agencourt AMPure XP 5 mL (Beckman Coulter Genomics, Cat # A63880)
Magnetic Stand -96 (Ambion, Cat # AM10027) / or / similar
2, 10, 20, 200 and 1000 L pipettes / multichannel pipettes
Nuclease-free barrier pipette tips
1.5 mL nuclease-free microcentrifuge tubes
Low melt agarose such as Low Gelling Temperature Agarose with a melt point of 65ºC (Boston Bioproducts, Cat # P-730) / or / E-gel system
1X TAE buffer
Clean razor or scalpel
SYBR Gold (Invitrogen, Cat # S11494)
UV transilluminator or gel documentation instrument
Gel electrophoresis apparatus
Electrophoresis power supply
Sequences of NEXTflex DNA Barcode Sequences for Ion Torrent - Excel / PDF