The NEXTflex™ 16S V4 Amplicon-Seq Kit is a validated amplicon-seq library prep kit developed to simplify bacterial metagenomics studies using Illumina® HiSeq and MiSeq platforms.
Fast, Two-Step Library Prep Protocol
The NEXTflex™ 16S V4 Amplicon-Seq Kit offers a fast two step, gel-free protocol that reduces library prep time to 2 hours and allows for complete automation of bacterial metagenomics studies. There are two main steps involved in the NEXTflex 16S V4 Amplicon-Seq Kit protocol: amplification and cleanup. Primers included in the NEXTflex 16S V4 Amplicon-Seq Kit are designed to hybridize to the fourth hypervariable (V4) domain of microbial 16S ribosomal RNA (rRNA) genes, allowing for amplification and sequencing of this variable region. The single cleanup step ensures maximum recovery of amplicons for downstream sequencing.
Users interested in different degrees of multiplexing should contact email@example.com.
The NEXTflex 16S V4 Amplicon-Seq Kit utilizes an excellent phylogenetic marker, allowing for studies of entire microbial communities or individual taxa, and is ideal for monitoring population fluctuations.
Bioo Scientific's NEXTflex™ 16S V1-V3 Amplicon-Seq Kit has recently been launched to enhance metagenomics studies.
Selected Publications that Reference using the NEXTflex 16S V4 Amplicon-Seq Kit:
Dalmassoa, A., et al. (2016) Characterization of microbiota in Plaisentif cheese by high-throughput sequencing. LWT - Food Science and Technology. 69, 490–496. doi:10.1016/j.lwt.2016.02.004.
Davitt, J. L., (2014) "An Assessment of Broiler Litter Bacterial Diversity using Next Generation DNA Sequencing." Master's Thesis, University of Tennessee, http://trace.tennessee.edu/utk_gradthes/3146.
Dees, M. W., Lysøe, E., Nordskog, B. and Brurberg, M. B. (2015) Bacterial Communities Associated with Surfaces of Leafy Greens: Shift in Composition and Decrease in Richness over Time. Appl. Envir. Microbiol., 81: 1530 - 1539. doi: 10.1128/AEM.03470-14.
Franchini P, Fruciano C, Frickey T, Jones JC, Meyer A (2014) The Gut Microbial Community of Midas Cichlid Fish in Repeatedly Evolved Limnetic-Benthic Species Pairs. PLoS ONE 9(4): e95027. doi:10.1371/journal.pone.0095027.
Liu, S., et al. (2016) The Host Shapes the Gut Microbiota via Fecal MicroRNA. Cell Host & Microbe. 19:1, 32–43. doi: 10.1016/j.chom.2015.12.005.
Lukens, J. R. et. al. (2014) Dietary modulation of the microbiome affects autoinflammatory disease. Nature. doi:10.1038/nature13788.
Lyons, P. P., Turnbull, J. F., Dawson, K. A. and Crumlish, M. (2016) Effects of low-level dietary microalgae supplementation on the distal intestinal microbiome of farmed rainbow trout Oncorhynchus mykiss (Walbaum). Aquaculture Research. doi: 10.1111/are.13080.
Lyons, P. P., Turnbull, J. F., Dawson, K. A. and Crumlish, M. (2015) Exploring the microbial diversity of the distal intestinal lumen and mucosa of farmed rainbow trout Oncorhynchus mykiss (Walbaum) using next generation sequencing (NGS). Aquaculture Research. doi: 10.1111/are.12863.
Man, S. M. et al. (2015) Critical Role for the DNA Sensor AIM2 in Stem Cell Proliferation and Cancer. Cell. doi:10.1016/j.cell.2015.06.001.
Martelli, F. S., et al. (2016) Long-term efficacy of microbiology-driven periodontal laser-assisted therapy. Eur J Clin Microbiol Infect Dis. 35:423–431. doi: 10.1007/s10096-015-2555-y.
Zeller, G. et al. (2014) Potential of fecal microbiota for early-stage detection of colorectal cancer. Mol Syst Biol. 10:766. doi:10.15252/msb.20145645.
The NEXTflex 16S V4 Amplicon-Seq Kit contains enough material to prepare 16, 48, 96, 192, 384, or 1,152 samples of bacterial genomic DNA for Illumina® compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C. This kit is shipped on dry ice.