NGS Library Prep and Multiplexing Kits


Bioo Scientific provides a complete portfolio of NGS library prep and multiplexing kits designed to increase the sensitivity, flexibility and speed of library prep for the IlluminaIon Torrent and SOLiD sequencing platforms. We offer complete kits for:


  • Whole Genome Sequencing: The identifications of the entire genome sequence of a given individual or organism
  • SNP Genotyping: The identification of variation in genetic sequences
  • Metagenome Analysis: Examination of bacterial populations from environmental samples
  • Epigenetics: Epigenetics is the study of chemical reactions that activate and deactivate parts of the genome at strategic times and in specific locations.
  • Gene Expression & Transcriptome Profiling: The analysis of the expression level of RNAs in a given cell or population of cells 

Our NGS library prep kits include an extensive line of NEXTflex™ library prep kits, NEXTflex™ barcoded adapters and NEXTprep™ nucleic isolation kits that have been optimized for sample and library preparation, critical steps in next generation sequencing. We have focused our attention on increasing enzymatic efficiency to achieve robust, high-quality sequencing data and cost effective solutions for next generation sequencing library preparation.


To meet the demands of our high throughput customers, NEXTflex reagents can be supplied in bulk quantities with custom formats. Bioo Scientific has an optimized inventory management system to ensure overnight delivery. We are committed to collaborating and working with customers who have unique research applications.

NGS News

$750,000 Awarded to Bioo Scientific to Continue Efforts toward Improving Small RNA-Seq Accuracy

Bioo Scientific Recognized at 2014 Greater Austin Business Awards

Bioo Scientific First to Address Small RNA-Seq Bias Concerns with Launch of New Kit

Bioo Scientific Launches 16S V1 – V3 rRNA Library Prep Kits for Bacterial Metagenomics Studies

Bioo Scientific Launches NEXTflex Cell Free DNA-Seq Kit Optimized for ctDNA and cfDNA Library Prep

Bioo Scientific Releases Sciclone and Biomek Protocols to Eliminate NGS Library Prep Bottlenecks

What's Next in NGS Blog

Reduced Library Prep Cost & Simplified Ordering with NEXTflex™ Bundles

Offering substantial cost-savings over our individual products, Bioo Scientific is introducing bundles of our most popular Illumina-compatible library prep kits.

Simplify Your 18S NGS Amplicon-Seq Studies with the New NEXTflex™ 18S ITS Amplicon-Seq Kit

Bioo Scientific released the NEXTflex™ 18S ITS Amplicon-Seq Kit, which simplifies the preparation of multiplexed amplicon libraries spanning the hypervariable Internal Transcribed Spacer (ITS) region of eukaryotic 18S ribosomal RNA (rRNA) genes for identification of uncultured fungal and micro-eukaryotic organisms.

Tech Tips: Bead-based Excess Adapter Depletion for Adapter-dimer Reduction in Reduced Bias Small RNA Libraries

The use of adapters with randomized ends, which have been incorporated into the NEXTflex Small RNA-Seq Kit v2, greatly reduces the ligase bias that exists in libraries prepared using traditional small RNA-Seq library prep protocols, which use non-randomized adapters.

Diet Dependent Microbiome Changes Protect Against Disease

Lukens, Gurung, Vogel, et. al. have recently uncovered diet-associated changes in the intestinal microbiome of mouse models as a significant factor regulating IL-1β maturation and the development of osteomyelitis. The researchers used the NEXTflex™ 16S V4 Amplicon-Seq Kit to characterize the organisms comprising the microbiomes of Pstpip2cmo mice that were fed a diet rich in fat and cholesterol.

Optimized Library Prep for Cell-Free DNA from Human Plasma

Researchers and clinicians are increasingly interested in using Next Generation Sequencing (NGS) analysis of cell-free DNA (cfDNA) found in plasma (the cell-free fraction of anticoagulated blood) for biomarker discovery and diagnostic applications.

Best Practices for Magnetic Bead Based Cleanups

The use of magnetic beads for DNA purification and size-selection offers a quick and high yield alternative compared to using column-based cleanups or gel purifications. We have compiled a number of suggestions to improve results for all four parts of magnetic bead based cleanups: adding beads, washing, resuspending and elution.

Use of RF and TRAP-RF Ribo-seq libraries for Precise Mapping of Ribosome Footprints to Study Translational Regulation in Arabidopsis thaliana

Piyada Juntawong, Thomas Girke, Jérémie Bazin, and Julia Bailey-Serres mapped ribosome footprints on Arabidopsis thaliana mRNAs to examine translational regulation under hypoxic stress conditions. RF and TRAP-RF Ribo-seq libraries were used to determine ribosome footprint number and position, in order to characterize posttranscriptional and translational control under normoxic and hypoxic conditions.


Optimized Library Preparation, an Essential Component of Next Gen Sequencing in Infectious Disease and Diagnostics

A Case Study of Rapid Metagenomic Diagnostics for Suspected Outbreaks of Severe Pneumonia

When: Tomorrow, January 20th at 9:00 am

Where: CDC, Clifton campus, Bldg 1-116/118

Sponsored by: Dr. Vladimir Loparev


North Carolina State University

Witherspoon Student Center

Monday, January 12, 2015

10:30 am - 1:30 am


Bioo Scientific's NGS library prep kit catalog