NGS Library Prep and Multiplexing Kits


Illumina Compatible NGS  Ion Compatible NGS  NGS Automation  Nucleic Acid Isolation


Bioo Scientific provides a complete portfolio of NGS library prep and multiplexing kits designed to increase the sensitivity, flexibility and speed of library prep for the Illumina and Ion Torrent sequencing platforms. We offer complete kits for:

  • Whole Genome Sequencing

    The identifications of the entire genome sequence of a given individual or organism

  • Targeted Resequencing:

    a highly targeted approach to analyzing specific genomic regions using amplicon panels

  • SNP Genotyping

    The identification of variation in genetic sequences

  • Metagenome Analysis

    The study of uncultured bacteria, fungus and micro-eukaryotic organisms

  • Epigenetics:  

    Epigenetics is the study of chemical reactions that activate and deactivate parts of the genome at strategic times and in specific locations.

  • Gene Expression & Transcriptome Profiling

    The analysis of the expression level of RNAs in a given cell or population of cells 

Our NGS library prep kits include an extensive line of NEXTflex™ library prep kits, NEXTflex™ barcoded adapters and NEXTprep™ nucleic isolation kits that have been optimized for sample and library preparation, critical steps in next generation sequencing. We have focused our attention on increasing enzymatic efficiency to achieve robust, high-quality sequencing data and cost effective solutions for next generation sequencing library preparation.

To meet the demands of our high throughput customers, NEXTflex reagents can be supplied in bulk quantities with custom formats. Bioo Scientific has an optimized inventory management system to ensure overnight delivery. We are committed to collaborating and working with customers who have unique research applications.

NGS News

$550,000 Awarded to Bioo Scientific to Enable the Improvement of Single Cell Sequencing Technologies

Bioo Scientific Granted Patent for Small RNA Sequencing Technology

Bioo Scientific’s Cell Free DNA-Seq Tech Now Manufactured under ISO 13485

Bioo Scientific Launches Completely Gel-Free or Low Input Small RNA Library Prep Kit

Bioo Scientific Releases Low-input, High Multiplexing Library Prep Solution for SeqCap Target Capture

Bioo Scientific Showcases Solutions Designed to Improve Exome Capture at ASHG 2015

Bioo Scientific Releases Automated Small RNA Library Prep Protocol for the Sciclone NGS Workstation

Bioo Scientific Increases Multiplexing Capabilities for Illumina Sequencing with >2,000 Single-Index Barcodes

Bioo Scientific Releases Automated NEXTflex Protocols for the Sciclone NGS Workstation

Bioo Scientific Releases Complete Library Prep Combo Kit to Improve SureSelectXT2 Target Capture

Bioo Scientific Partners with the Icahn School of Medicine at Mount Sinai to Reduce Ligation Bias in Next Generation Sequencing

Bioo Scientific Launches Kit Offering Robust, Automation-friendly cfDNA Isolation

What's Next in NGS Blog

What Are the Biological Processes that Contribute to Cell-free DNA in the Circulation of Healthy Individuals?

Recently, there has been renewed interest in understanding the origins of DNA found in the cell-free fraction of blood (i.e. plasma) and other body fluids. Analysis of cfDNA by NGS and other methods is being used for monitoring recurrence of malignancy in cancer patients undergoing treatment, as well as for non-invasive prenatal diagnostics and other clinical applications.

Using the NEXTflex Small RNA-Seq Kit v3 for RIP-Seq, CLIP-Seq and Ribosomal Profiling

A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments.

Analysis Recommendations for DNA-Seq

The advancements and decreased cost of high-throughput sequencing provide researchers with access to a multitude of genetic data. Based on specific experimental goals, methods, and pipeline tools, the extent of data analysis required can be highly variable. Some bioinformatics packages integrate easily into central analysis pipelines, while other tools are more optimized for use as standalone packages.

Detecting Germline Mutations

Mendelian diseases, also known as monogenic diseases, represent a class of disorders associated with genetic variation in one gene. Their inheritance follows classic Mendelian laws, and the phenotype could be expressed in either a dominant or recessive way. Currently, over three thousands genes have been associated with Mendelian diseases including cystic fibrosis, cancer predisposition, epilepsy, and deafness among others.

Bioo Scientific’s Cell Free DNA-Seq Tech Now Manufactured under ISO 13485

To meet the needs of pre-clinical and clinical scientists and to improve traceability and quality control, Bioo Scientific’s NEXTflex™ Cell Free DNA-Seq Kits for the construction of libraries for cell free DNA sequencing are now manufactured under ISO 13485 certification.

NGS Meeting & Tradeshow


Jun 1 - 3, 2016

Santa Fe, NM


Development of Amplicon Panels for Testing of Inherited Mendelian Diseases

Wednesday, June 1  5:55 pm

A Fast and Reliable Amplicon-based NGS Strategy for Screening of Germline and Somatic Mutations in BRCA1 and BRCA2 Genes

Wednesday, June 1  6:10 pm

ASM Microbe 2016

Jun 16 - 20, 2016

Boston, MA

Booth # 904