Bioo Scientific Launches Completely Gel-Free or Low Input Small RNA Library Prep Kit
Bioo Scientific Releases Low-input, High Multiplexing Library Prep Solution for SeqCap Target Capture
Bioo Scientific Showcases Solutions Designed to Improve Exome Capture at ASHG 2015
Bioo Scientific Releases Automated Small RNA Library Prep Protocol for the Sciclone NGS Workstation
Bioo Scientific Increases Multiplexing Capabilities for Illumina Sequencing with >2,000 Single-Index Barcodes
Bioo Scientific Releases Automated NEXTflex Protocols for the Sciclone NGS Workstation
Bioo Scientific Releases Complete Library Prep Combo Kit to Improve SureSelectXT2 Target Capture
Bioo Scientific Partners with the Icahn School of Medicine at Mount Sinai to Reduce Ligation Bias in Next Generation Sequencing
Bioo Scientific Launches Kit Offering Robust, Automation-friendly cfDNA Isolation
What's Next in NGS Blog
New Amplicon Panel Offering Complete BRCA1 and BRCA2 Coverage for Illumina Sequencing
The NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for Illumina Sequencing is a cost-effective, optimized solution for revealing clinically relevant mutations in BRCA1 and BRCA2 genes by detecting and screening DNA isolated from fresh or frozen tissue.
Advantages of Using Amplicon Panels for Targeted Resequencing
Targeted sequencing is an invaluable tool in clinical and research applications methods to sequence region(s) of interest. Two principally different technologies exist for enrichment of library pools for targeted sequencing – target capture by hybridization and amplicon sequencing (Samorodnitski et al., 2015).
Slideshow – Reduced Bias Small RNA Library Prep with Gel-Free or Low-Input Options
microRNAs (miRNAs) may provide useful markers for the development of disease diagnostic and prognostic assays. NGS brings sensitivity, specificity, and the ability to maximize data acquisition and minimize costs of miRNA sequencing by using multiplex strategies to allow many samples to be sequenced simultaneously with small RNA analysis.
Addressing Bias in Small RNA Library Preparation for Sequencing: A New Protocol Recovers microRNAs that Evade Capture by Current Methods
Researchers from the University of North Carolina at Chapel Hill, the National Human Genome Research Institute, and the NIH Intramural Sequencing Center, recently published their research, “Addressing bias in small RNA library preparation for sequencing: a new protocol recovers microRNAs that evade capture by current methods,” in the journal Frontiers in Genetics.
Several issues have historically reduced the utility of small RNA sequencing, including bias introduced during ligation, tedious gel purification requirements, and high input requirements. Bioo Scientific’s newest whitepaper, Reduced-bias small RNA library preparation with gel-free or low-input options, illustrates how the NEXTflex Small RNA-Seq Kit v3 solves these issues, offering sensitivity, specificity, and the ability to maximize data acquisition and minimize costs.