NGS Library Prep and Multiplexing Kits


Illumina Compatible NGS  Ion Compatible NGS  NGS Automation  Nucleic Acid Isolation


Bioo Scientific provides a complete portfolio of NGS library prep and multiplexing kits designed to increase the sensitivity, flexibility and speed of library prep for the Illumina and Ion Torrent sequencing platforms. We offer complete kits for:

  • Whole Genome Sequencing

    The identifications of the entire genome sequence of a given individual or organism

  • Targeted Resequencing:

    a highly targeted approach to analyzing specific genomic regions using amplicon panels

  • SNP Genotyping

    The identification of variation in genetic sequences

  • Metagenome Analysis

    The study of uncultured bacteria, fungus and micro-eukaryotic organisms

  • Epigenetics:  

    Epigenetics is the study of chemical reactions that activate and deactivate parts of the genome at strategic times and in specific locations.

  • Gene Expression & Transcriptome Profiling

    The analysis of the expression level of RNAs in a given cell or population of cells 

Our NGS library prep kits include an extensive line of NEXTflex™ library prep kits, NEXTflex™ barcoded adapters and NEXTprep™ nucleic isolation kits that have been optimized for sample and library preparation, critical steps in next generation sequencing. We have focused our attention on increasing enzymatic efficiency to achieve robust, high-quality sequencing data and cost effective solutions for next generation sequencing library preparation.

To meet the demands of our high throughput customers, NEXTflex reagents can be supplied in bulk quantities with custom formats. Bioo Scientific has an optimized inventory management system to ensure overnight delivery. We are committed to collaborating and working with customers who have unique research applications.

NGS News

Bioo Scientific Launches Completely Gel-Free or Low Input Small RNA Library Prep Kit

Bioo Scientific Releases Low-input, High Multiplexing Library Prep Solution for SeqCap Target Capture

Bioo Scientific Showcases Solutions Designed to Improve Exome Capture at ASHG 2015

Bioo Scientific Releases Automated Small RNA Library Prep Protocol for the Sciclone NGS Workstation

Bioo Scientific Increases Multiplexing Capabilities for Illumina Sequencing with >2,000 Single-Index Barcodes

Bioo Scientific Releases Automated NEXTflex Protocols for the Sciclone NGS Workstation

Bioo Scientific Releases Complete Library Prep Combo Kit to Improve SureSelectXT2 Target Capture

Bioo Scientific Partners with the Icahn School of Medicine at Mount Sinai to Reduce Ligation Bias in Next Generation Sequencing

Bioo Scientific Launches Kit Offering Robust, Automation-friendly cfDNA Isolation

What's Next in NGS Blog

New Amplicon Panel Offering Complete BRCA1 and BRCA2 Coverage for Illumina Sequencing

The NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for Illumina Sequencing is a cost-effective, optimized solution for revealing clinically relevant mutations in BRCA1 and BRCA2 genes by detecting and screening DNA isolated from fresh or frozen tissue.

Advantages of Using Amplicon Panels for Targeted Resequencing

Targeted sequencing is an invaluable tool in clinical and research applications methods to sequence region(s) of interest. Two principally different technologies exist for enrichment of library pools for targeted sequencing – target capture by hybridization and amplicon sequencing (Samorodnitski et al., 2015).

Slideshow – Reduced Bias Small RNA Library Prep with Gel-Free or Low-Input Options

microRNAs (miRNAs) may provide useful markers for the development of disease diagnostic and prognostic assays. NGS brings sensitivity, specificity, and the ability to maximize data acquisition and minimize costs of miRNA sequencing by using multiplex strategies to allow many samples to be sequenced simultaneously with small RNA analysis.

Addressing Bias in Small RNA Library Preparation for Sequencing: A New Protocol Recovers microRNAs that Evade Capture by Current Methods

Researchers from the University of North Carolina at Chapel Hill, the National Human Genome Research Institute, and the NIH Intramural Sequencing Center, recently published their research, “Addressing bias in small RNA library preparation for sequencing: a new protocol recovers microRNAs that evade capture by current methods,” in the journal Frontiers in Genetics.

Reduced-Bias Small RNA Library Preparation with Gel-Free or Low-Input Options

Several issues have historically reduced the utility of small RNA sequencing, including bias introduced during ligation, tedious gel purification requirements, and high input requirements. Bioo Scientific’s newest whitepaper, Reduced-bias small RNA library preparation with gel-free or low-input options, illustrates how the NEXTflex Small RNA-Seq Kit v3 solves these issues, offering sensitivity, specificity, and the ability to maximize data acquisition and minimize costs.

NGS Meeting & Tradeshow


Feb 10 - 13, 2016

Orlando, FL


Feb 20 - 23, 2016

Fort Lauderdale, FL

Booth # 227

Molecular-Med TRICON

Mar 6 - 11, 2016

San Francisco, CA

Booth # 128


Apr 16 - 20, 2016

New Orleans, LA

Booth # 850