NGS Library Prep and Multiplexing Kits


Illumina Compatible NGS  Ion Compatible NGS  NGS Automation  Nucleic Acid Isolation


Bioo Scientific provides a complete portfolio of NGS library prep and multiplexing kits designed to increase the sensitivity, flexibility and speed of library prep for the Illumina and Ion Torrent sequencing platforms. We offer complete kits for:

  • Whole Genome Sequencing

    The identifications of the entire genome sequence of a given individual or organism

  • Targeted Resequencing:

    a highly targeted approach to analyzing specific genomic regions using amplicon panels

  • SNP Genotyping

    The identification of variation in genetic sequences

  • Metagenome Analysis

    The study of uncultured bacteria, fungus and micro-eukaryotic organisms

  • Epigenetics:  

    Epigenetics is the study of chemical reactions that activate and deactivate parts of the genome at strategic times and in specific locations.

  • Gene Expression & Transcriptome Profiling

    The analysis of the expression level of RNAs in a given cell or population of cells 

Our NGS library prep kits include an extensive line of NEXTflex™ library prep kits, NEXTflex barcoded adapters and NEXTprep™ nucleic isolation kits that have been optimized for sample and library preparation, critical steps in next generation sequencing. We have focused our attention on increasing enzymatic efficiency to achieve robust, high-quality sequencing data and cost effective solutions for next generation sequencing library preparation.

To meet the demands of our high throughput customers, NEXTflex reagents can be supplied in bulk quantities with custom formats. Bioo Scientific has an optimized inventory management system to ensure overnight delivery. We are committed to collaborating and working with customers who have unique research applications.

NGS News


Bioo Scientific Launches BRCA1/2 Amplicon Panel for Detection of Somatic Mutations in FFPE Samples

$550,000 Awarded to Bioo Scientific to Enable the Improvement of Single Cell Sequencing Technologies

Bioo Scientific Granted Patent for Small RNA Sequencing Technology

Bioo Scientific’s Cell Free DNA-Seq Tech Now Manufactured under ISO 13485

Bioo Scientific Launches Completely Gel-Free or Low Input Small RNA Library Prep Kit

Bioo Scientific Releases Low-input, High Multiplexing Library Prep Solution for SeqCap Target Capture

Bioo Scientific Showcases Solutions Designed to Improve Exome Capture at ASHG 2015

Bioo Scientific Releases Automated Small RNA Library Prep Protocol for the Sciclone NGS Workstation

Bioo Scientific Increases Multiplexing Capabilities for Illumina Sequencing with >2,000 Single-Index Barcodes

Bioo Scientific Releases Automated NEXTflex Protocols for the Sciclone NGS Workstation

Bioo Scientific Releases Complete Library Prep Combo Kit to Improve SureSelectXT2 Target Capture

Bioo Scientific Partners with the Icahn School of Medicine at Mount Sinai to Reduce Ligation Bias in Next Generation Sequencing

Bioo Scientific Launches Kit Offering Robust, Automation-friendly cfDNA Isolation

What's Next in NGS Blog


The NEXTflex™ Bisulfite-Seq Kit uses a similar workflow to other DNA-Seq library preparation kits, but with the addition of a bisulfite conversion step. This conversion takes place following adapter ligation, and consists of denaturing and chemically converting all non-methylated cytosines into uracils.

BRCA1/2 Amplicon Panel Offers Best-in-class Performance for Detection of Mutations in FFPE Samples

The NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE lllumina Sequencing can be used to detect clinically relevant somatic mutations in DNA isolated from FFPE samples, to facilitate variant discovery and confirmation. This kit is a cost-effective, optimized solution offering best-in-class performance for on-target percentage and coverage uniformity.

Bioo Scientific’s NGS Marketing Collateral Places in Top International Award

Out of more than 5,000 submissions from 21 countries, Bioo Scientific is named a Silver winner in the 2016 Summit Creative Award® competition for its 2016 NGS Calendar which was entered in the Specialty Item category.

Validated, Custom Amplicon Panels for Illumina and Ion Torrent Sequencing

The NEXTflex™ Custom Amplicon Panels are fully customizable amplicon-based assays for targeted resequencing on the Illumina or Ion Torrent sequencing platforms.

What Are the Biological Processes that Contribute to Cell-free DNA in the Circulation of Healthy Individuals?

Recently, there has been renewed interest in understanding the origins of DNA found in the cell-free fraction of blood (i.e. plasma) and other body fluids. Analysis of cfDNA by NGS and other methods is being used for monitoring recurrence of malignancy in cancer patients undergoing treatment, as well as for non-invasive prenatal diagnostics and other clinical applications.

Using the NEXTflex Small RNA-Seq Kit v3 for RIP-Seq, CLIP-Seq and Ribosomal Profiling

A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments.