NextPrep-Mag™ Urine cfDNA Isolation Kit 

For isolation of circulating DNA from urine for NGS

Catalog# Product Name Quantity US List Price
NOVA-3826-01 NextPrep-Mag™ Urine cfDNA Isolation Kit (< 1 mL - 4 mL) 12 - 50 isolations $155 Buy Now
NOVA-3826-02  NEXTprep-Mag™ Urine cfDNA Isolation Kit (4 mL – > 20 mL) 5 - 25 isolations $206 Buy Now
         
NOVA-5150-01 NEXTflex® Cell Free DNA-Seq Kit for Illumina Library Prep
8 rxns $303 Buy Now
NOVA-5150-02 NEXTflex® Cell Free DNA-Seq Kit for Illumina Library Prep
48 rxns $1,362 Buy Now
NOVA-4002-01 NEXTflex® Cell Free DNA-Seq Kit for Ion Torrent Library Prep
8 rxns $176 Buy Now
NOVA-4002-02 NEXTflex® Cell Free DNA-Seq Kit for Ion Torrent Library Prep 48 rxns $835 Buy Now

NextPrep-Mag Urine cfDNA Isolation Kit
  • Ideal for extracting DNA from urine for cell-free DNA analysis
  • Generates highly concentrated libraries using few PCR cycles
  • Automation friendly, mag-bead based format
  • Fast, 30 minute protocol with minimal hands-on time
  • Ideal for NGS library prep
  • No vacuum pumps, vacuum manifolds, or column extenders required

The NextPrep-Mag™ Urine cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) for constructing NGS libraries from urine, using a fast magnetic bead-based format requiring minimal hands-on time. The isolated cell free DNA is ideal for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing


Cell Free DNA Isolation Protocol

The procedure, which can be completed in approximately 30 minutes, includes an initial lysis and DNA binding step, wash steps, and elution of cfDNA from the magnetic beads. A distinguishing feature of the kit is the very rapid magnetic bead attraction steps.

NextPrep-Mag™ Urine cfDNA Isolation Kit Protocol


NextPrep-Mag Urine cfDNA Isolation Kit Compared to the QIAamp™ Circulating Nucleic Acid Kit 

The NextPrep-Mag Urine cfDNA Isolation Kit offers a number of advantages over the QIAamp™ Circulating Nucleic Acid Kit, including speed of cfDNA isolation and ease of automation, while delivering comparable cfDNA yields and higher concentration NGS libraries.

NextPrep-Mag versus QIAamp


Kit Specs

The NextPrep-Mag Urine cfDNA Isolation Kit For < 1 mL - 4 mL contains sufficient reagents for extraction of DNA from 50 samples of 1 mL urine or less, or 12 samples of 4 mL of urine. The NextPrep-Mag Urine cfDNA Isolation Kit For 4 mL - > 20 mL contains sufficient reagents for extraction of DNA from 25 samples of 4 mL urine or less, or 5 samples of 20 mL of urine. The kit components are stable for at least 12 months from time of receipt, when properly stored.

Kit Contents

cfDNA DNA Proteinase K

Urine cfDNA Binding Solution

cfDNA Magnetic beads               

cfDNA Wash Solution 1

cfDNA Wash Solution 2

cfDNA Elution Solution

Control PCR Primers

 

Required Materials Not Provided

Ethanol, 100%

Vortex mixer

Heat block at 55°C

Magnetic stands to hold 2 mL microcentrifuge tubes and 15 mL or 50 mL tubes

Microcentrifuge or low-speed “picofuge”

Nuclease-free 2 mL (1.5 mL and 0.5 mL optional) microcentrifuge tubes and 15 mL tubes

Pipettors (P-1000, P-200, P-20) and tips

Disposable pipettes (variable sizes depending on sample volume processed)