ELISA for the Screening of Food for Nitrofuran Residues
The MaxSignal® Nitrofuran group of products provide competitive ELISA kits for the quantitative analysis of each nitrofuran in a variety of sample types.
Nitrofurans are antibiotics frequently used in animal production due to their highly efficient antibacterial properties. These drugs are also used as growth promoters during the production of shrimp, poultry, pigs, and others. However, the parent compounds and their metabolites have been shown to have carcinogenic and mutagenic characteristics which led to the banning of nitrofurans for the treatment of animals used for food production. In 1993, the EU banned the nitrofurans furaltadone, nitrofurantoin and nitrofurazone for use in animals used as sources of food, and in 1995 the use of furazolidone was also banned.
The MaxSignal® technology is based on a competitive colorimetric ELISA assay where the antibody has been coated in the plate wells. During the analysis, sample is added along with target specific HRP conjugate and if the target residue is present in the sample, it will compete for the antibody, thereby preventing the HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the TMB substrate, has an inverse relationship with the target drug residue concentration in the sample.
This kit is manufactured to the international quality standard ISO 9001:2015.
PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter Plate Reader with 450 nm wavelength (Bioo Cat# 6003-01)
STANDARDS (PPB): AMOZ, SEM, AHD - 0 / 0.025 / 0.1 / 0.4 / 1.6 / 6.4
AOZ - 0 / 0.02 / 0.06 / 0.18 / 0.54 / 1.62
TESTS PER KIT: The kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards).
STORAGE: Store all kit components at 2 – 8°C. Certain components should be stored at - 20°C if longer storage is required. See kit manual for more details.
Publications that Reference MaxSignal® Nitrofuran ELISA Kits
Contia, G. O., Chiara Copata, C., Wangb, Z., D’Agatia, P., Cristaldia, A. and Ferrantea, M. (2014) Determination of illegal antimicrobials in aquaculture feed and fish: An ELISA study. Food Control. Doi: 10.1016/j.foodcont.2014.10.050