- Rapid, 10 - 30 minute, cost-effective extraction methods that offer high recovery rates
- High sensitivity - 0.125 ppb
- Low detection limit - 30 ppb for mussel samples and 6 ppb for water samples
- Fast - less than 1.5 hours regardless of number of samples
- High reproducibility
Rapid and Sensitive Domoic ELISA
The MaxSignal® Domoic Acid (ASP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Domoic Acid in mussels, algae and water samples. This kit is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Domoic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the Domoic Acid-horseradish peroxidase conjugate (Domoic Acid-HRP Conjugate) and Anti- Domoic Acid Antibody. If the Domoic Acid residue is present in the sample, it will compete with Domoic Acid-HRP Conjugate for the Anti-Domoic Acid Antibody, thereby preventing the Domoic Acid-HRP Conjugate from binding to the Anti-Domoic Acid Antibody. The Anti-Domoic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the domoic acid residue concentration in the sample.
This kit is manufactured to the international quality standard ISO 9001:2008.
Domoic acid and its isomers are water-soluble neurotoxins produced by a number of marine algae, in particular by the microalgae of the genus Pseudo-nitzschia. Blooms of Pseudo-nitzschia spp. may lead to the accumulation of domoic acid in shellfish filter feeders and other marine species. Ingestion of domoic acid contaminated shellfish may cause human illness called amnesic shellfish poisoning (ASP) by affecting the central nervous system. ASP has caused the death of both animal and human consumers in severe cases. The European Commission Directive 2002/226/EC implemented a domoic acid maximum permitted level (MPL) of 20 ppm (mg/kg) in shellfish intended for human consumption. This MPL is adopted by the regulatory authorities in most other countries.
Publications that Cite the Use of the MaxSignal Domoic Acid ELISA Test Kit
Johnson, S., Harrison, H. and Turner, A. D. (2016) Application of rapid test kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain. Toxicon, 111:1. 121-129. doi:10.1016/j.toxicon.2016.01.052.
McLeod, C., Burrell, S. and Holland, P. (2015) Review of the currently available field methods for detection of marine biotoxins in shellfish flesh.
The MaxSignal Domoic Acid (ASP) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.