Sensitive ELISA for Detection of Okadaic Acid in Mussel and Water Samples
The MaxSignal® Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of okadaic acid in mussels and water samples. Okadaic acid (OA) is one of the diarrheic shellfish poisons (DSP) produced by the dinoflagellate genera Dinophysis and Prorocentrum. There are a number of chemically different toxins associated with DSP. They are lipophilic and polyether compounds and can be divided into three main groups:
- Acidic toxins - OA and its derivatives
- Dinophysistoxins (DTX1, DTX2, DTX3)
- Neutral toxins - pectenotoxin group (PTXs)
The MaxSignal Okadaic Acid (DSP) ELISA Test Kit is a competitive colorimetric ELISA assay. The toxin has been coated in the plate wells. During analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Contamination of shellfish with OA has been associated with harmful algal blooms throughout the world. In humans, DSP causes dose-dependent symptoms of diarrhea, nausea and vomiting. The action level established by the FDA for OA is 0.2 ppm. The EU has established a level of 160 µg/kg of OA or its equivalents.
This kit is manufactured to the international quality standard ISO 9001:2008.
Publications that Cite the Use of the MaxSignal Okadaic Acid (DSP) ELISA Test Kit
Baker, S. and Götz, J. (2016) A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathologica Communications Neuroscience of Disease. 4:32. doi: 10.1186/s40478-016-0300-0.
Johnson, S., Harrison, K. and Turner, A.D. (2016) Application of Rapid Test Kits for the determination of Diarrhetic Shellfish Poisoning (DSP) toxins in bivalve molluscs from Great Britain, Toxicon. doi: 10.1016/j.toxicon.2016.01.052.
McLeod, C., Burrell, S. and Holland, P. (2015) Review of the currently available field methods for detection of marine biotoxins in shellfish flesh.
Turner, A. D. and Goya, A. B. (2016) Comparison of four rapid test kits for the detection of okadaic acid-group toxins in bivalve shellfish from Argentina. Food Control. 59. 829–840. doi: 10.1016/j.foodcont.2015.07.005.
PLATE: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate
STANDARDS: 0 / 0.3 / 0.9 / 2.7 / 5.4 / 16.2
SHELF LIFE & STORAGE: 12 months when properly stored.