Rapid and simple method
- Minimal sample prep
- Highly accurate and reproducible
Blood Urea Nitrogen Enzymatic Kit for Determination of Urea in Serum
The MaxDiscovery™ Blood Urea Nitrogen Enzymatic Kit is a microplate-based colorimetric assay for the determination of urea in serum samples produced from blood. Blood urea nitrogen (BUN) is an important marker for normal kidney and liver function. Elevation of BUN levels is often an indication of intestinal and kidney obstruction and cardiac failure. Decreased BUN levels are often associated with kidney and liver damage. BUN is also a very useful tool for preclinical investigation of experimental drug formulations and BUN levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents.
MaxDiscovery Blood Urea Nitrogen Enzymatic Kit uses an enzyme-based assay to determine urea in liquid samples such as serum. The test is based on a highly proven method for urea determination. The MaxDiscovery Blood Urea Nitrogen Enzymatic Kit contains sufficient
materials to test 42 samples in duplicate.
The assay utilizes urease, a metabolic enzyme, to specifically detect urea in serum. The MaxDiscovery Blood Urea Nitrogen Enzymatic Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 8 ppm urea for serum. The
linear range of the assay is 8 – 200 ppm analyte.
Selected Publications that Reference the Use of the MaxDiscovery BUN Assay Kit:
Ahn T.G., et al. (2013) Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone. Biochemical Pharmacology 85 (2013) 1525–1533.
Das, S., et al. (2016) Tissue deposition and toxicological effects of commercially significant rare earth oxide nanomaterials: Material and physical properties. Environmental Toxicology. doi: 10.1002/tox.22290.
Harrill, A.H. et al. (2012) A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models. Toxicological Sciences. 130 (2): 416-426.
Jyothi, K. R. et al. (2015) Liver-targeted cyclosporine A-encapsulated poly (lactic-co-glycolic) acid nanoparticles inhibit hepatitis C virus replication. International Journal of Nanomedicine. 10, 903–921.
Patlolla, A. K., Randolph, J., Kumari, S. A. and Tchounwou, P. B. (2016) Toxicity Evaluation of Graphene Oxidein Kidneys of Sprague-Dawley Rats. Int. J. Environ. Res. Public Health. 13:4. 380. doi: 10.3390/ijerph13040380.
Ramani, K., et al. (2014) An essential role of interleukin-17 receptor signaling in the development of autoimmune glomerulonephritis. Journal of Leukocyte Biology 95(6). doi: 10.1189/jlb.3A0414-184R.
Zhu, D., Lam, D. H., Purwanti, Y. I., Goh, S. L., Wu, C., Zeng, J., Fan, W. and Wang, S. (2013) Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells. Mol. Ther. 21:8, 1621-30.
MaxDiscovery Blood Urea Nitrogen Kit Specs
The MaxDiscovery Blood Urea Nitrogen Enzymatic Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit at 4°C. The shelf life is 6 months when the kit is properly stored.