High sensitivity with low detection limit (25 mg/dl)
- Robust kinetic assay
- Rapid – results in 10 minutes
- Does not require expensive instrumentation
- Highly reproducible results
- Measure absorbance in visual spectrum (520 nm)
Cholesterol Assay Kit for the Determination of Cholesterol in Serum Samples
The MaxDiscovery™ Cholesterol Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of cholesterol in serum samples.
The MaxDiscovery Cholesterol Enzymatic Assay Kit uses a coupled enzymatic reaction scheme: cholesterol esters are first converted to cholesterol and fatty acids. Next, cholesterol is oxidized with O2 to form cholesten-3-one + H2O2. Lastly, the hydrogen peroxide is reacted with 4-aminoantipyrine and p-HBS to yield quinoneimine (red dye) and water. The absorption measured at 520 nm, is proportional to the concentration of cholesterol in the sample. The kit also comes with a control solution containing a cholesterol standard (500 mg/dl) which can be used to calibrate the assay.
Selected Publications that Reference the Use of the MaxDiscovery Cholesterol Enzymatic Assay Kit
Berezina, A. E., Kremzerb, A. A., Berezinac, T. A., and Martovitskayad, Y. V. (2016) The pattern of circulating microparticles in patients with diabetes mellitus with asymptomatic atherosclerosis. Acta Clinica Belgica: International Journal of Clinical and Laboratory Medicine. doi:10.1080/17843286.2015.1110894.
FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter plate reader (520 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate
SHELF LIFE: 3 months when stored under optimal conditions
SAMPLE SIZE: 10 μL/well
SAMPLE TYPES: Rodent Serum