High sensitivity and low detection limit (10 mg/dL)
- A rapid (10 minutes) and robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
HDL Cholesterol Assay for the Determination of HDL Cholesterol in Rodent Serum and Plasma
The MaxDiscovery™ HDL Cholesterol Assay Kit is a plate-based colorimetric assay for the determination of HDL cholesterol in serum or plasma samples. The kit uses a spectrophotometric assay to detect HDL directly from serum samples, enabling researchers to measure HDL cholesterol levels in rodent serum.
High-density lipoprotein (HDL) is one of the five major groups of lipoproteins which enables lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. HDL particles are primarily involved in the uptake and transport of cholesterol from peripheral tissue to the liver for excretion or re-utilization. This reverse cholesterol transport is a proposed cardio protective mechanism, as low levels of HDL cholesterol have been linked to increased risk of coronary heart disease and coronary artery disease. For this reason, measuring HDL cholesterol levels has been shown to often identify those at a greater risk for heart disease.
The MaxDiscovery HDL Cholesterol Assay Kit is a simple microplate-based method for measuring HDL cholesterol levels from serum or plasma. This kit uses a specific reagent formulation to selectively stabilize non-HDL lipoprotein particles (LDL, VLDL and chylomicrons) while leaving HDL particles untouched. Next a second reagent containing a detergent and modified enzymes selectively reacts with the cholesterol present only in the HDL particles to form hydrogen peroxide. The hydrogen peroxide product then reacts with N-(2-hydroxy-3-sulfopropyl)-3,5-dimethyoxyaniline to form a colored product. The resulting color change is measured at 610 nm and is proportional to the amount of HDL cholesterol originally present in the sample.
The kit also comes with a control solution containing a HDL cholesterol standard which can be used to calibrate the assay and generate a standard curve.
Publications that Cite the Use of the MaxDiscovery HDL Cholesterol Assay:
Evans, J.F. et al. (2012) The lipocalin-type prostaglandin D2 synthase knock-out (L-PGDS KO) mouse model of insulin resistance and obesity demonstrates early hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. Journal of Endocrinology. doi: 10.1530/JOE-12-0275.
Miranda, C. L., et al. (2016) Xanthohumol improves dysfunctional glucose and lipid metabolism in diet-induced obese C57BL/6J mice. Archives of Biochemistry and Biophysics. doi:10.1016/j.abb.2016.03.008.
MaxDiscovery HDL Cholesterol Assay Kit Specs
The MaxDiscovery HDL Cholesterol Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit as described in the table below. The shelf life is 6 months after receipt when the kit is properly stored.