Increased sensitivity with low detection limit (10 U/L)
- Convenient, colorimetric, end-point assay
- Does not require expensive instrumentation
- Highly reproducible results
- Requires only 10 µL of serum
- Measure absorbance in visual spectrum (510 nm)
ALT Assay for the Determination of the Alanine Transaminase Enzyme in only 10 µL of Serum
The MaxDiscovery™ Alanine Transaminase (ALT) Color Endpoint Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the alanine transaminase enzyme in 10 µL of serum from rodents or other mammals. Alanine transaminase (ALT) also known as alanine aminotransferase or (sGPT) is a metabolic enzyme expressed primarily in the liver. Elevation of ALT levels is an indication of liver damage and has been associated with liver injury. ALT levels are monitored routinely in patients with liver diseases. ALT is also a very useful tool for preclinical investigation of experimental drug formulations and ALT levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.
Selected Publications that Cite Use of the MaxDiscovery Alanine Transaminase (ALT) Color Endpoint Assay Kit:
Alakeel, R.A. (2012) Iron overload in chronic Hepatitis C patients with Candida albicans infection. African Journal of Microbiology Research Vol. 6(32), pp. 6210-6217. doi: 10.5897/AJMR12.1017.
Cai, P., Gobert, G. N., You, H., Duke, M. and McManus, D. P. (2015) Circulating miRNAs: Potential Novel Biomarkers for Hepatopathology Progression and Diagnosis of Schistosomiasis Japonica in Two Murine Models. PLOS Neglected Tropical Diseases. doi: 10.1371/journal.pntd.0003965.
Das, S., et al. (2016) Tissue deposition and toxicological effects of commercially significant rare earth oxide nanomaterials: Material and physical properties. Environmental Toxicology. doi: 10.1002/tox.22290.
De Filette, M. et. al. (2014) Vaccination of Mice Using the West Nile Virus E-Protein in a DNA Prime-Protein Boost Strategy Stimulates Cell-Mediated Immunity and Protects Mice against a Lethal Challenge. PLOS ONE. doi: 10.1371/journal.pone.0087837.
Endo H, Niioka M, Kobayashi N, Tanaka M, Watanabe T (2013) Butyrate-Producing Probiotics Reduce Nonalcoholic Fatty Liver Disease Progression in Rats: New Insight into the Probiotics for the Gut-Liver Axis. PLoS ONE 8(5): e63388. doi:10.1371/journal.pone.0063388
Hanson, M.S., Xu, H. et al. (2012) A novel hemoglobin-binding peptide reduces cell-free hemoglobin in murine hemolytic anemia. American Journal of Physiology. Vol. 304 No. 2 H328-H336. doi: 10.1152/ajpheart.00500.2012
Mitra, A., Satelli, A., Yan, J., Xueqing, X., Gagea, M., Hunter, C. A., Mishra, L. and Li, S. (2014), IL-30 (IL27p28) attenuates liver fibrosis through inducing NKG2D-rae1 interaction between NKT and activated hepatic stellate cells in mice. Hepatology. doi: 10.1002/hep.27392.
Zhu, D., Lam, D. H., Purwanti, Y. I., Goh, S. L., Wu, C., Zeng, J., Fan, W. and Wang, S. (2013) Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells. Mol. Ther. 21:8, 1621-30.
MaxDiscovery ALT Color Endpoint Assay Kit Specs
The MaxDiscovery ALT Color Endpoint Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 6 months when properly stored.