High sensitivity with low detection limit (20 U/L)
- Rapid – results in 5 minutes
- Robust kinetic assay
- Does not require expensive instrumentation
- Highly reproducible results
- Only requires 10 µL of serum
- Measure absorbance in UV spectrum (340 nm)
ALT Assay for the Determination of Alanine Transaminase in Serum
The MaxDiscovery™ Alanine Transaminase (ALT) Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of alanine transaminase enzyme in 10 µL of serum from rodents or other mammals. Alanine transaminase (ALT) (also known as alanine aminotransferase or sGPT) is a metabolic enzyme expressed primarily in the liver. Damage to the liver causes the release of this enzyme into the blood. Elevation of ALT levels is an indication of liver damage and has been associated with liver injury. ALT levels are monitored routinely in patients with liver diseases. ALT is also a very useful tool for preclinical investigation of experimental drug formulations and ALT levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.
Selected References Which Cite the Use of the MaxDiscovery ALT Enzymatic Assay:
Fang X, Wang R, Ma J, Ding Y, Shang W, et al. (2012) Ameliorated ConA-Induced Hepatitis in the Absence of PKC-theta. PLoS ONE 7(2): e31174. doi:10.1371/journal.pone.0031174
Carlisle, R, et. al. (2013) Enhanced tumor uptake and penetration of virotherapy using polymer stealthing and focused ultrasound. J. Natl. Cancer Inst. 105:1701-10
Goraczniak R, Wall BA, et al. (2013) U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo. Molecular Therapy Nucleic Acids (2013) 2, e92; doi:10.1038/mtna.2013.24.
Hila, E. F., et al. (2015) Quercetin tests negative for genotoxicity in transcriptome analyses of liver and small intestine of mice. Food and Chemical Toxicology. doi:10.1016/j.fct.2015.04.005.
Kühnel, F. et al. (29 December 2009) Targeting of p53-Transcriptional Dysfunction by Conditionally Replicating Adenovirus Is Not Limited by p53-Homologues. Mol. Ther.
Wu, S.Y. et al. (12 August 2010) Systemic delivery of E6/7 siRNA using novel lipidic particles and its application with cisplatin in cervical cancer mouse models. Gene Therapy.
Kverka, M. et al. (May 2011) Safety and efficacy of the immunosuppressive agent 6-tioguanine in murine model of acute and chronic colitis, BMC Gastroenterology, 11 (1) 47.
Hu, S. et al. (July 6, 2011) 13C-Pyruvate Imaging Reveals Alterations in Glycolysis that Precede c-Myc-Induced Tumor Formation and Regression. Cell Metabolism 14 (1): 131 – 142.
Patel R, Baker SS, Liu W, Desai S, Alkhouri R, et al. (2012) Effect of Dietary Advanced Glycation End Products on Mouse Liver. PLoS ONE 7(4): e35143. doi:10.1371/journal.pone.0035143.
van der Stelta, I. et.al (2015) Nutraceutical oleuropein supplementation prevents high fat diet-induced adiposity in mice. Journal of Functional Foods. 14, 702–715. doi:10.1016/j.jff.2015.02.040.
MaxDiscovery ALT Enzymatic Assay Kit Specs
The MaxDiscovery Alanine Transaminase (ALT) Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 12 months when properly stored. Once the Reagent Mix is reconstituted the shelf life of the kit is 3 months when properly stored.