High sensitivity with low detection limit
- Robust kinetic assay
- Does not require expensive instrumentation
- Highly reproducible results
- Requires only 10 µL of serum
- Measure absorbance in visual spectrum (405 nm)
AP Enzymatic Assay for the Determination of Alkaline Phosphatase in Serum
The MaxDiscovery™ Alkaline Phosphatase (AP) Enzymatic Assay Kit is a plate-based, colorimetric-endpoint assay for the determination of alkaline phosphatase enzyme in 10 µL of serum from rodents or other mammals.
MaxDiscovery Alkaline Phosphatase Enzymatic Assay Kit uses an enzymatic assay to determine the amount of alkaline phosphatase in rodent serum and other liquid samples. This alkaline phosphatase assay enables biomedical researchers to determine alkaline phosphatase levels in liquid samples such as serum. The MaxDiscovery Alkaline Phosphatase Enzymatic Assay Kit contains sufficient materials to test 42 samples in duplicate.
The assay utilizes a simple colorimetric (visible) enzymatic assay to specifically detect AP in fluids. The MaxDiscovery AP Assay Kit provides accurate, proven results even in complex liquid mixtures. The kit is designed to be used with a microplate reader. The alkaline phosphatase assay kit contains a thymolphthalein standard to construct a linear calibration curve which can be used to verify assay performance.
Alkaline phosphatase (AP) is a ubiquitously-expressed intracellular enzyme which catalyzes the hydrolysis of organic phosphoesters. AP is a clinically important protein marker in serum because its level changes in response to a number of health-related states. For example, changes in AP serum levels are often caused by liver and liver and bone diseases. Also, the presence of elevated levels of the enzyme in serum after administration of drugs and experimental therapeutic agents is associated with organ toxicity. Therefore, monitoring serum levels of AP enzyme has become a routine method to monitor drug toxicity.
Selected References Which Cite the Use of the MaxDiscovery AP Enzymatic Assay Kit:
Goraczniak R, Wall BA, et al. (2013) U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo. Molecular Therapy Nucleic Acids (2013) 2, e92; doi:10.1038/mtna.2013.24.
Latarya, G. et al. (2012) Human Aqueous Humor Phosphatase Activity in Cataract and Glaucoma. Investigative Ophthalmology & Visual Science. doi: 10.1167/iovs.11-9120
Invest. Ophthalmol. Vis. Sci. March 26, 2012 vol. 53 no. 3 1679-1684
MaxDiscovery AP Enzymatic Assay Kit Specs
The MaxDiscovery Alkaline Phosphatase Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for serially-diluted standards). Upon receipt of the kit, place the dilution buffer at -20°C. Store the remainder of the kit at 4°C. The shelf life is 6 months when the kit is properly stored.