High sensitivity and low detection limit (10 U/L)
- A rapid (20 min), robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
- Only requires 5 µL of serum
Aspartate Transaminase Assay Kit for the Determination of the Aspartate Transaminase in Serum
The MaxDiscovery™ Aspartate Transaminase (AST) Color Endpoint Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in 5 µL of serum from rodents or other mammals. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed primarily in the liver. Elevation of AST levels is an indication of liver damage and has been associated with liver injury. AST levels are monitored routinely in patients with liver diseases. AST is also a very useful tool for preclinical investigation of experimental drug formulations and AST levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.
This Alanine Transaminase Assay Kit uses a colored reaction scheme to detect AST enzymatic activity in serum samples. Using this AST assay kit, aspartate and α-ketoglutarate are first converted to glutamate and oxaloacetate. The oxaloacetate then reacts with diazonium salt to form a colored product. The concentration of AST in each sample is then directly determined from the absorbance at 510 nm measured with a plate reader. Dilutions of the oxaloacetate control, included in the kit, can be used to construct a standard curve to calibrate the assay and confirm assay linearity.
Selected Publications that Reference the Use of the MaxDiscovery AST Color Endpoint Assay Kit:
Alakeel, R.A. (2012) Iron overload in chronic Hepatitis C patients with Candida albicans infection. African Journal of Microbiology Research Vol. 6(32), pp. 6210-6217. DOI: 10.5897/AJMR12.1017.
Cai, P., Gobert, G. N., You, H., Duke, M. and McManus, D. P. (2015) Circulating miRNAs: Potential Novel Biomarkers for Hepatopathology Progression and Diagnosis of Schistosomiasis Japonica in Two Murine Models. PLOS Neglected Tropical Diseases. doi: 10.1371/journal.pntd.0003965.
Naowaboot, J., Wannasiri, S. and Pannangpetch, P. (2016) Morin attenuates hepatic insulin resistance in high-fat-diet-induced obese mice. Journal of Physiology and Biochemistry. pp 1-12.
Zhu, D., Lam, D. H., Purwanti, Y. I., Goh, S. L., Wu, C., Zeng, J., Fan, W. and Wang, S. (2013) Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells. Mol. Ther. 21:8, 1621-30.
MaxDiscovery AST Color Endpoint Assay Kit Specs
The MaxDiscovery Aspartate Transaminase Color Endpoint Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. The shelf life of the kit is 6 months, after receipt, when the kit is properly stored.